不同来源滇白珠中木脂素苷的含量测定

目的 :测定滇白珠中木脂素苷的含量 ,探讨评价滇白珠质量的方法。方法 :高效液相色谱法。色谱柱为KromasilC18(0 .4cm× 2 5cm) ,流动相为甲醇 乙腈 水 (30∶5∶6 5 ) ,波长 220nm ,外标法。结果 :进样量与峰面积线性关系良好 (D₁,D₂ ,D₄ 分别为r₁=0.9997,r₂ =0.9999和r₄=0.9999)。D₁平均回收率为 98.9% ,RSD 2.6%。不同产地药材木脂素苷 (LD)含量不同 ,贵阳、广西金秀县和昆明LD平均测定值分别是 :208.8,158.2,154.8mg·(100g)^-1。小范围采集样品LD含量基本稳定 ,其变化幅度明显小于不同分布地区的差异。嫩茎比枯老茎LD总含量高1倍 ,采收时留嫩去老方可保证疗效。结论 :此方法简便、可靠 ,可以作为评价滇白珠质量的参考方法。

Determination of Lignan Glycosides in Gaultheria leucocarpa var. yunnanensis by RP-HPLC

OBJECTIVE: To control the quality of Gaultheria leucocarpa var. yunnanensis the contents of lignans in these plants from different provinces were detected. METHOD: HPLC was adopted using Hypersil-18 column (0.4 cm x 25 cm), methanol: acetonitrile: water (30:5:65) as mobile phase. RESULT: The calibration curves of D1(gaultherinA), D2(-)-5'-methoxyisolariciresinol-2 alpha-O-beta-D-xylopyranoside)], D4(+)-lyoniresinol-2 alpha-O-beta-D-glucopyranoside)] were linear in the range of 0.10-0.40 microgram, 0.03-0.13 microgram, 0.02-0.09 microgram, respectively. The average recovery of D1 was 98.9% and RSD was 2.6%. CONCLUSION: Comparing the samples from Jinxiou(158.2 mg.100 g)-1 and Kumming(154.8 mg.100 g)-1, that of Guiyang showed the highest content of LD(208.8 mg.100 g)-1. The content of LD in the tender above-ground part was 1.3 times higher than that in the wilt.