基于临床用药实际的不同状态下仙茅多次给药后血药浓度变化

目的: 观察仙茅作用于正常、虚寒两种不同状态大鼠后,其入血成分苔黑酚葡萄糖苷的血药浓度变化差异,探讨仙茅在正常与虚寒状态下体内药代与生物学效应的相关性。 方法: SD雄性大鼠随机分为两组,即正常组和虚寒组。连续14 d肌注氢化可的松粉针剂20 mg·kg^-1·d^-1塑造虚寒大鼠模型。模型成功后,正常/虚寒大鼠连续7 d给予仙茅水提液30 g·kg^-1,最后1次给药后,分别于多点动物交叉眼眶取血,采用高效液相法检测苔黑酚葡萄糖苷血药浓度,并计算药物代谢参数。 结果: 虚寒组大鼠在给药后20 min,其血药浓度高于正常组大鼠,直到120 min达最大血药浓度,其高水平状态一直维持到300 min;且虚寒组大鼠达峰时间(T_max)、血药浓度-时间曲线下面积(AUC)明显升高(P<0.05)。 结论: 虚寒组大鼠体内苔黑酚葡萄糖苷浓度始终处于高水平状态,可能为其生物学效应的更好发挥提供了物质基础。

Plasma Concentration of Curculiginis Rhizoma on Different States of Clinical Practice

Objective: To observe the plasma concentration of orcinol glucoside of Curculiginis Rhizoma in blood on normal and deficiency cold state in rats, to explore that the correlation of Curculigini Rhizoma metabolize and and biological effects. Method: Male SD rats were randomly divided into two groups, namely the normal group and the deficiency cold group. The deficiency cold rats were given hydrocortisone sodium succinate injection(20 mg·kg^-1·d^-1) by intramuscular for 14 days. From the 15th day Curculigini Rhizoma(30 g·kg^-1·d^-1) water extracts was given ig to two groups rats for 7 days. After the last time, blood was collected for detecting plasma concentration of orcinol glucoside at multiple time points by HPLC, and calculate metabolic parameters. Result: The plasma concentration was increased in deficiency cold rats at 20 min, plasma concentration reached the maximum at 120 min, high level was maintained to 300 min;time to maximum plasma concentration(T_max) and area under the curve(AUC) were increased significantly(P<0.05). Conclusion: The plasma concentration is high level in deficiency cold rats, that may provide the material basis for biological effect of Curculiginis Rhizoma.