灯盏细辛-赤芍组方配比及给药途径的脑保护作用

目的: 研究灯盏细辛与赤芍不同组方配比及给药途径对大鼠局灶性脑缺血的脑保护作用。 方法: 线栓法制作大鼠中动脉缺血再灌注损伤模型。SD大鼠随机分为假手术组、模型组、阳性药物组、灯盏细辛与赤芍不同组方配比给药组。各组动物按各自不同的途径(iv,ig)给药,在预定时间测定神经行为学、脑梗死率等7个关键生物学指标。结果采用析因实验设计及分析方法(2×4),通过对各因素(途径、配方)下各个水平(4个不同配比处方)的全部组合进行实验。用SPSS 13.0统计学软件对数据进行分析处理,分析考察因素及其交互作用对指标影响的显著性,同时进一步考察各因素下水平间的显著性差异。 结果: 60%赤芍组静脉注射给药,对拟适应症相关的神经行为学、脑梗死率等7个关键生物学指标具有统计学意义。 结论: 60%赤芍组静脉注射给药,对局灶性脑缺血再灌注损伤大鼠有一定的脑保护作用。

Effect of Erigerontis Herba and Paeoniae Radix Rubra Compound with Different Proportion and Different Routes of Administration on Brain Damage in Rat Model of Cerebral Focal Ischemia and Reperfusion

Objective: To investigate the effect of Erigerontis Herba and Paeoniae Radix Rubra compound with different proportion and two different routes of administration(intravenous and oral) on brain damage in the rat model of cerebral focal ischemia and reperfusion. Method: SD rats were divided into four groups randomly, normal control group,ischemia-reperfusion group,positive control group and different proportion of Erigerontis Herba and Paeoniae Radix Rubra compound group, 16 in each group. The Longa method was used to establish the MCAO model. Paeoniae Radix Rubra compound at different proportion was injected or given by gavage at different time points according to the test design. Based on factorial experiment design and factor analysis method, the whole combinations of each factors(route, proportion) and each levels(four different ratio of formula) were designed. The influence significance of each factors and their interaction for indexes were analyzed. Also,the significant difference of all the levels of every factor was observed. Result: The compatibility of Erigerontis Herba and Paeoniae Radix Rubra at a ratio of 1:1.5 was found, which could obviously improve pivotal biological indicator,and include neuroethology, the rate of cerebral infraction, and so on. Conclusion: The compatibility ratio of Erigerontis Herba and Paeoniae Radix Rubra of is 1:1.5, which exerts a protective effect on cerebral ischemia-reperfusion injury.