镇肝熄风汤对帕金森病模型小鼠神经行为学及黑质14-3-3、酪氢酸羟化酶表达的影响

目的: 观察镇肝熄风汤对1-甲基-4-苯基-1,2,3,6-四氢吡啶(1-methy-4-phenyl-1,2,3,6-tetrahy-dropyridine MPTP)诱导的C57BL/6小鼠帕金森病模型自主活动的影响及其可能机制。 方法: 连续灌胃14 d给予不同剂量的镇肝熄风汤(12.5, 25,50 g·kg^-1)和司来吉兰(10 mg·kg^-1)预处理,于给药第11天开始连续4 d C57BL/6小鼠皮下注射MPTP(30 mg·kg^-1)制备帕金森病小鼠模型。通过自主活动实验 观察动物行为学表现,采用实时定量RT-PCR法检测多功能蛋白14-3-3和酪氨酸羟化酶(tyrosine hydroxylase, TH) mRNA表达,Western blot方法检测多功能蛋白14-3-3,TH蛋白表达。 结果: 与模型组比较,镇肝熄风汤可显著增强帕金森病小鼠自主活动能力(均P<0.05),上调多功能蛋白14-3-3和TH mRNA和蛋白表达(均P<0.05)。 结论: 镇肝熄风汤具有抗实验性帕金森病的作用,其机制可能与上调多功能蛋白14-3-3和TH的mRNA和蛋白表达有关。

Effect of Zhengan Xifeng Decoction on Expression of 14-3-3 and Tyrosine Hydroxylase Gene in Substantia Nigra of MPTP-treated C57BL/6 Mice

Objective: The aim of this study was to investigate the effects of Zhengan Xifeng decoction on the spontaneous movement in 1-methy-4-phenyl-1,2,3,6-tetrahy-dropyridine(MPTP)-treated C57BL/6 mice, and some mechanisms involved. Method: C57BL/6 mice were given MPTP (30 mg·kg^-1) to prepare Parkinson disease mice model. Different doses of Zhengan Xifeng decoction (12.5, 25, 50 g·kg^-1), or selegiline (10 mg·kg^-1) were given for 14 d in the pretreatment groups. The behavioral analysis of C57BL/6 mice was performed by using spontaneous movement test. Real-time PCR and Western blot were used to analyze the expression of 14-3-3 and tyrosine hydroxylase (TH) mRNA and protein. Result: Zhengan Xifeng decoction could reduce the number of spontaneous movement of MPTP-treated C57BL/6 mice. Zhengan Xifeng decoction could also up-regulate the expression of 14-3-3 and TH mRNA and protein. Conclusion: Anti-Parkinson disease effect of Zhengan Xifeng decoction may be attributable to up-regulation mRNA and protein expression of 14-3-3 and TH in MPTP-treated C57BL/6 mice.