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| 嗅鞘细胞对活化的星形胶质细胞的作用 | |
| 引用本文： | 刘志元,刘锦波,张志坚,龚爱华,张俊.嗅鞘细胞对活化的星形胶质细胞的作用[J].江苏大学学报(医学版),2010,20(2):131-135,I0001. |
| 作者姓名： | 刘志元 刘锦波 张志坚 龚爱华 张俊 |
| 作者单位： | 1. 南京医科大学附属常州二院中心实验室,江苏,常州,213003 2. 常州市第一人民医院骨科,江苏,常州,213003 3. 江苏大学医学院,江苏,镇江,212013 |
| 基金项目： | 江苏省医学重点人才资助项目，江苏省卫生厅科教兴卫工程 |
| 摘 要： | 目的:研究体外培养的大鼠嗅鞘细胞(olfactory ensheathing cell,OEC)对活化的星形胶质细胞(astrocytes,AST)的作用.方法:体外培养嗅鞘细胞和星形胶质细胞.星形胶质细胞经纯化传代,细胞融合后利用划伤法得到活化的星形胶质细胞,将嗅鞘细胞与活化的星形胶质细胞共培养24 h后,观察星形胶质细胞增殖能力的改变,以及其表达胶质纤维酸性蛋白(GFAP)和硫酸软骨素蛋白多糖(CSPG)的变化.结果:活化的星形胶质细胞与嗅鞘细胞共培养后,MTT实验表明其增殖能力增强;免疫荧光染色、RT-PCR和蛋白质印迹法测定结果表明共培养后活化的星形胶质细胞表达的GFAP和CSPG降低.结论:嗅鞘细胞作用于活化后的星形胶质细胞以后,能够促进星形胶质细胞的分裂增殖,同时能够减少星形胶质细胞胶质化,降低抑制性细胞因子的表达. |
| 关 键 词： | 嗅鞘细胞 星形胶质细胞 增殖 胶质纤维酸性蛋白(GFAP) 硫酸软骨素蛋白多糖(CSPG) |
Effect of olfactory ensheathing cells on reactive astrocytes |
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| LIU Zhi-yuan,LIU Jin-bo,ZHANG Zhi-jian,GONG Ai-hua,ZHANG Jun.Effect of olfactory ensheathing cells on reactive astrocytes[J].Journal of Jiangsu University Medicine Edition,2010,20(2):131-135,I0001. | |
| Authors: | LIU Zhi-yuan LIU Jin-bo ZHANG Zhi-jian GONG Ai-hua ZHANG Jun |
| Institution: | LIU Zhi-yuan1,LIU Jin-bo2,ZHANG Zhi-jian3,GONG Ai-hua3,ZHANG Jun1(1.Central Lab,the Second Hospital of Changzhou Affiliated of NJMU,Changzhou Jiangsu 213003,2.Department of Orthopaedics,the First People\'s Hospital of Changzhou,3 School of Medicine,Jiangsu University,Zhenjiang Jiangsu 212013,China) |
| Abstract: | Objective:To study the effect of olfactory ensheathing cells on reactive astrocytes in vitro.Methods:Olfactory ensheathing cells and astrocytes were cultured from Sprague Dawley Rats in vitro.Astrocytes were purified then passaged and grown to confluence.Some cultures were scratched to induce a reactive astrocyte phenotype.Olfactory ensheathing cells were added onto confluent astrocytes for 24 h.The differences of cell proliferation,expressions of glial fibillary acidic protein(GFAP) and chondroitin sulfate... |
| Keywords: | olfactory ensheathing cells astrocytes proliferation GFAP CSPG |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |