| 小鼠釉基质丝氨酸蛋白酶成熟肽编码区cDNA的克隆和序列分析 |
| |
| 引用本文： | 顾淑萍,费俭等. 小鼠釉基质丝氨酸蛋白酶成熟肽编码区cDNA的克隆和序列分析[J]. 牙体牙髓牙周病学杂志, 2001, 11(4): 210-212 |
| |
| 作者姓名： | 顾淑萍 费俭等 |
| |
| 作者单位： | [1]第四军医大学口腔医学院,陕西西安710032 [2]中国科学院细胞生物学研究所,上海200233 |
| |
| 基金项目： | 国家自然科学基金资助项目 (3 980 0 15 5 ) |
| |
| 摘 要： | 目的：克隆小鼠釉基质丝氨酸蛋白酶（EMSP）成熟肽编码区的基因。方法：设计引物,以小鼠牙胚总RNA为模板,利用RT-PCR方法,扩增出小鼠EMSP的基因片段,将所得基因片段插入pBS质粒载体。转化到大肠杆菌XL-1-Blue后挑选阳性克隆,提取重组质粒DNA,通过限制性酶切和核苷酸序列分析鉴定阳性克隆。结果：重组质粒pBS-EMSP的酶切图谱和序列分析结果与国外文献报道一致。结论：克隆到小鼠EMSP成熟肽编码区基因。
|
| 关 键 词： | 小鼠 RT－PCR 釉基质丝氨酸蛋白酶 |
Cloning of mouse enamel matrix serine proteinase encoding mature protein |
| |
| GU Shu-ping,LIU Han,SHI Jun-nan,et al. Cloning of mouse enamel matrix serine proteinase encoding mature protein[J]. Chinese Journal of Conservative Dentistry, 2001, 11(4): 210-212 |
| |
| Authors: | GU Shu-ping LIU Han SHI Jun-nan et al |
| |
| Abstract: | AIM:To obtain the cDNA sequence encoding mature protein of mouse EMSP. METHOD: The desired DNA product was obtained from the total RNA of mouse dental germ by RT-PCR with two gene specific primers. The segment (about 800bp) was inserted into pBlueScript vector, and the interesting plasmid was transformed into E. Coli host strain XL-1-Blue. The double-stranded DNA of the postitive clone was analyzed by restriction endonuclease mapping and DNA sequencing. RESULT: The restriction endonuclease map and sequence of mouse EMSP encoding mature protein were consistent with those of the references appeared. CONCLUSION: The mouse EMSP cDNA encoding mature protein was obtained for further study. [ |
| |
| Keywords: | mouse RT-PCR enamel matrix seerine proteinase(EMSP) |
| 本文献已被 CNKI 维普 等数据库收录！ |
