抗SARS-CoV免疫球蛋白乳酸-羟乙酸共聚物微球的制备与性质

 目的制备抗SARS-CoV免疫球蛋白(ASI)乳酸-羟乙酸共聚物(PLGA)微球,研究其体外释放性质及中和SARS活性。方法采用复乳溶剂挥发法制备ASI微球,以激光粒度测定仪测定微球的平均粒径,以BCA法及微量BCA法测定微球的蛋白含量及蛋白从微球的释放。用ELISA法考察从微球中释放出的ASI的活性。结果ASI微球粒径均匀,平均粒径为15.87 μm,蛋白包裹率为70.1%,载药量为6.3%,微球中包裹的ASI与原蛋白溶液相比活性不损失。结论采用复乳溶剂挥发法,通过控制一定的因素,可以得到具有较高包封率的ASI微球。

Preparation and characteristics of anti-SARS-CoV immunoglobulin poly (lactide-co-glycolide acid) microspheres

OBJECTIVE To prepare Anti-SARS-CoV immunoglobulinn (ASI) PLGA microspheres and to study on the release characteristics in vitro and the activity of neutralization of ASI on SARS virus.METHODS The microspheres were prepared by using (water-in-oil)- in-water emulsion solvent evaporation technique.The microspheres diameter was determined by laser particle sizer. The concentration of ASI and the release of ASI from microspheres were measured by BCA and micro BCA method.The neutralization activity of ASI release from micro-spheres was evaluated by ELISA method.RESULTS ASI microspheres were homogeneous with the average diameter of 15.87 μm,the encapsulation of protein was 70.1% and protein content was 6.3%, the activity of ASI release from microspheres was maiteined. CONCLUSION The microspheres containing relatively high concentration of ASI can be produced by controling different process.