益气明目口服液对光损伤模型大鼠的疗效观察

目的:探讨中药益气明目口服液对实验性视网膜光损伤模型大鼠的治疗作用。方法:Sprague-Dawley大鼠54只随机分为阴性对照组、光损伤模型组、益气明目口服液组。采用绿色荧光灯(Lux为1900±106.9)24h持续光照射建立大鼠视网膜急性光损伤模型,光照前分别采用等量蒸馏水和益气明目口服液灌胃治疗7d。光照后6d,观察视网膜电图的改变,检测视网膜组织中超氧化物歧化酶(SOD)的活性及丙二醛(MDA)的含量,并进行视网膜透视电子显微镜和光学显微镜的组织学观察。结果:光损伤后6d,益气明目口服液对实验大鼠视网膜光损伤后ERGa波及b波振幅的降低均有保护作用,益气明目口服液组视网膜中SOD的活性高于光损伤模型组(P<0.01),而MDA的含量则低于光损伤模型组(P<0.01);通过视网膜透视电子显微镜和光学显微镜的组织学观察,益气明目口服液组的病理组织学损害较光损伤模型组明显减轻。结论:益气明目口服液是一种治疗视网膜光损害性疾病的有效中药复方制剂。

Effects of Yiqimingmu Solution on Models of Light Damage in Rat Retina

PURPOSE: To investigate the experimental therapeutic effects of Chinese medicine Yiqimingmu solution on rats of retina injury models induced by light. METHODS: Fifty-four Sprague-Dawley rats were randomly divided into negative control group, light damage model group and Yiqimingmu solution group. The light damage model was induced by exposing the SD rats' light eyes to the light of green fluorescent (intensity = 1900 +/- 106.9 Lux) for 24 hours. Distilled water and Yiqimingmu solution were respectively irrigated into rats for 7 days before light expose. In 6 days after light expose, the change of electroretinogram (ERG) was observed. The content of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) in rats retina were measured, and the histopathologic changes were observed with transmission electron microscopy and light microscopy. RESULTS: In 6 days after light expose, Yiqimingmu solution had protective effects on fall of ERG a wave and b wave amplitude induced by light damage. The activity of SOD in Yiqimingmu solution group was higher than in light damage model group (P < 0.01= , the content of MDA in Yiqimingmu solution group was lower than in light damage model group (P < 0.01). The histopathologic injury of rat retina in Yiqimingmu solution group was less than in light damage model group by means of transmission electron microscopy and light microscopy. CONCLUSIONS: Yiqimingmu solution is an effective traditional Chinese medicine to treat diseases of retina light damage.