Demonstration of the Philadelphia translocation by fluorescence in situ hybridization (FISH) in paraffin sections and identification of aberrant cells by a combined FISH/immunophenotyping approach |
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| Authors: | M. NOLTE M. WERNER M. EWIG R. VON WASIELEWSKI L. WILKENS A. GEORGII |
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| Affiliation: | Pathologisches Institut der Medizinischen Hochschule Hannover, Hannover, Germany |
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| Abstract: | The Philadelphia translocation was demonstrated by two-colour fluorescence in situ hybridization (FISH) in decalcified paraffin sections of bone marrow from patients with chronic myelogenous leukaemia. FISH was combined with immunocytochemical detection of different membrane-bound or cytoplasmic antigens. With this new technique, the cells bearing the 9;22 translocation can be identified morphologically, as well as immunocyto-chemically, in tissue sections. |
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| Keywords: | chronic myelogenous leukaemia Philadelphia translocation fluorescence in situ hybridization (FISH) |
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