宁夏道地药材苦豆子醇提取物体外抑菌作用研究

目的：观察宁夏苦豆子65%乙醇提取物对细菌和真菌的体外抑菌作用。方法：将苦豆子药用65%乙醇浸泡24 h后,回流两次所得提取物,用试管二倍稀释法联合琼脂平板法,进行体外抑菌试验的研究。结果：苦豆子65%乙醇提取物对三种常见致病菌及一种深部真菌抑菌作用显著,其最小抑菌浓度（MIC）均为35.1 mg/mL。结论：苦豆子中草药用65%乙醇提取物对四种受试菌的抑菌作用显著。

Study on the Antimicrobial Activity from Ethanol-extract of Ningxia Sophora Alopecuroides in Vitro

Objective： To observe the antimicrobial activity of 65% ethanol-extract of Ningxia Sophora alopecuroides on the bacteria and fungus in vitro.Methods： Soak the Sophora alopecuroides in the 65% ethanol after 24 h,and gain the antimicrobial test of the extract by refluxing twice,which was performed by the tube double dilution method combined with agar plate method in vitro.Results： 60% ethanol-extract of Ningxia Sophora alopecuroides showed the distinct bacteriostasis effect on the three common pathogenic bacteria and one species of deep fungus;the minimum inhibitory concentration（MIC） were 35.1 mg/mL.Conclusion：65% ethanol-extract of herb medicine Sophora alopecuroides has the distinct effect of bacteriostasis on the four bacteria.