重组HMGB1 A-box蛋白对HMGB1抗炎作用的观察

目的：观察重组HMGB1 A-box蛋白对HMGB1的特异性拮抗作用。方法：复苏的RAW264.7细胞培养至对数生长期,用于以下实验：实验分为六组,r HMGB1组：加500 ng/mL r H-MGB1;LPS组：加100 ng/mL LPS;A-box干预1组：加500 ng/mL r HMGB1和100 ng/mL A-box;A-box干预2组：加500 ng/mL r HMGB1和200 ng/mL A-box;A-box干预3组：加500 ng/mL r HMGB1和500 ng/mL A-box;A-box干预4组：加100 ng/mL LPS和500 ng/mL A-box。培养6 h后,收集上清液,待测。各组上清液采用ELISA盒检测TNF-α的含量。结果：200 ng/mL、500 ng/mL重组A-box蛋白能明显抑制HMGB1诱导培养细胞释放TNF-α的水平（P〈0.05）,但对LPS的无明显作用。结论：r HMGB1 A-box蛋白可能有特异性拮抗HMGB1的致炎作用。

The Role of rHMGB1 A-box Protein Inhibiting HMGB1-induced Proinflammation

Objective： To observe rHMGB1 A-box protein specific antagonist HMGB1. Methods： The recovery RAW264. 7 cells were cultured until the cells on logarithmic phase were prepared for the experiment. The trials are divided into six groups, rHMGB1 group： 500 ng/mL rHMGB1 ; LPS group： 100 ng/mL LPS; A-box intervention group 1 ： 500 ng/ mL rHMGB1 and 100 ng/mL A-box; A-box intervention group 2：500 ng/mL rHMGB1 and 200 ng/mL A-box;A-box intervention group 3：500 ng/mL rHMGB1 and 500 ng/mL A-box;A-box intervention group 4：100 ng/mL LPS and 500 ng/mL A-box. Each group cells were stimulated for 6 h, then the levels of TNF-α in the supernatant were measured by ELISA. Results： 200 ng/mL,500 ng/mL rHMGB1 A-box protein could significantly inhibit HMGBl-induced TNF-α production （ P〈0. 05）, but LPS did not. Conclusion： rHMGB1 A-box protein could specifically antagonize proinflammatory role of HMGB1.