山东省沂蒙山区莱姆病自然疫源地调查

目的 :调查山东省沂蒙山区莱姆病自然疫源地。方法 :1992～ 1999年设点 ,用间接免疫荧光抗体试验进行人群莱姆病感染调查 ;用直接免疫荧光抗体法进行蜱中肠带螺旋体率调查 ;取鼠的肾脏、膀胱分离病原。结果 :沂蒙山区人群莱姆病平均感染率为 7.50 % ,林区人群感染率明显高于非林区人群 ;该地区蜱类以长角血蜱为优势种 ,该蜱中肠携带莱姆病螺旋体率为 12 .0 % ,从86组长角血蜱培养物中获得 2株莱姆病螺旋体。结论 :从病原学上证实沂蒙山区为莱姆病自然疫源地

An Endemic Investigation of Lyme Disease in Yimeng Mountains,Shandong Province

From 1992 to 1999 an endemic investigation of Lyme disease was conducted by means of serological and etiological methods in Yimeng mountains of Shandong province. Of 695 forestry residents,49 residents had a significant antibody titer against Borrelia burgdorferi strain B 31 .The prevalance rate of Lyme disease was 7.50 %. Haemaphysalis longicornis was a prevailing species of Ixodidae in the region.Spirochetes were observed in the midguts of 50 H.longicornis in this area by the direct fluorescence antibody method.The positive rate was 12.0 %(6/50).2 strains (TSH 1,TSH 3) of spirochetes were isolated from H.longicornis .Both TSH 1 and TSH 3 were positively reacted to McAb H 5332 and H 9724 ,but negatively to H 6831 .It was confirmed that Yimeng mountains of Shandong province was the endemic area of Lyme disease.