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| 曲古菌素A对视网膜母细胞瘤Hxo-rb44细胞凋亡及Survivin、c-Myc表达的影响 | |
| 引用本文： | 王国军,胡燕华,李鹏程,徐茜. 曲古菌素A对视网膜母细胞瘤Hxo-rb44细胞凋亡及Survivin、c-Myc表达的影响[J]. 郑州大学学报(医学版), 2006, 41(2): 324-326 |
| 作者姓名： | 王国军 胡燕华 李鹏程 徐茜 |
| 作者单位： | 1. 华中科技大学同济医学院附属协和医院眼科,武汉,430022;郑州大学医学院,郑州,450052 2. 华中科技大学同济医学院附属协和医院眼科,武汉,430022 3. 华中科技大学同济医学院附属同济医院肿瘤生物医学中心,武汉,430022 |
| 摘 要： | 目的:探讨曲古菌素A(TSA)对人视网膜母细胞瘤(Rb)细胞株Hxo-rb44细胞凋亡和Survivin、c-Myc表达的影响.方法:将人Rb细胞株Hxo-rb44进行培养传代,流式细胞仪检测不同浓度的TSA作用不同时间对Hxorb44细胞周期和凋亡的影响,用Western blot法检测凋亡过程中Survivin和c-Myc蛋白表达的变化.结果;TSA可明显诱导细胞发生G2期阻滞及细胞凋亡,而且与药物作用时间呈依赖关系(r=0,97,P=0,02);Western blot法可以观察到Survivin和c-Myc的表达水平随着TSA作用时间的延长而下降(P均＜0,01).结论:TSA可以有效诱导人Rb细胞株Hxo-rb44细胞周期阻滞和凋亡,其作用机制与抑制Survivin和c-Myc表达有关. |
| 关 键 词： | 曲古菌素A 视网膜母细胞瘤 Hxo-rb44细胞 细胞周期阻滞 凋亡 Survivin c-Myc |
| 收稿时间： | 2005-12-29 |
| 修稿时间： | 2005-12-29 |
Effect of trichostatin A on apoptosis and expression of Survivin and c-Myc of retinoblastoma cell Hxo-rb44 |
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| WANG Guojun,HU Yanhua,LI Pengcheng,XU Qian. Effect of trichostatin A on apoptosis and expression of Survivin and c-Myc of retinoblastoma cell Hxo-rb44[J]. Journal of Zhengzhou University: Med Sci, 2006, 41(2): 324-326 | |
| Authors: | WANG Guojun HU Yanhua LI Pengcheng XU Qian |
| Affiliation: | 1. Department of Ophthalmology, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022; 2. Medical College, Zhengzhou University, Zhengzhou 450052 ;3. Biological Medical Center of Tumors, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022 |
| Abstract: | Aim: To investigate the effect of trichostatin A (TSA) on apoptosis and expression of Survivin and c-Myc of retinoblastoma cell Hxo-rb44.Methods:The retinoblastoma cell Hxo-rb44 was chosen in this experiment. The cell cycle arrest and apoptosis of Hxo-rb44 cell were assayed with flow cytometry. Expression of Survivin and c-Myc was detected by Western blot. Results: TSA obviously induced cell cycle arrest and apoptosis in time-dependent manner (r=0.97,P= 0.02 ). The expression of Survivin and c-Myc decreased with TSA incubation time (P<0.01).Conclusion: TSA can effectively induce cell cycle arrest and apoptosis in Hxo-rb44 cell, which may associate with TSA suppressing the expression of survivin and c-Myc. |
| Keywords: | trichostatin A retinoblastoma Hxo-rb44 cell cell cycle arrest apoptosis Survivin c-Myc |
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