利用骨髓涂片检测白血病微小残留病

以抗原受体基因重排为标志，通过多聚酶链反应（PCR）检测急性淋巴细胞白血病（ALL）微小残留病（MRD），近年来在国内外已较广泛地开展起来。但以往的检测方法多是从新鲜骨髓液或外周血液中提取细胞DNA模板，用于PCR扩增，不仅取材不便，标本的保存、送检有困难，DNA抽提方法也较繁锁。我们最近从骨髓涂片中提取DNA，通过PCR检测MRD，取得较好效果，现报告如下。骨髓涂片中DNA的提取方法一、主要度剂和仪器从骨髓涂片中提取DNA所需试剂少，仪器简单，主要有：NIDPK缓冲液（50mMKCl；10mMTris-Cl，pH8．3；2．5mMgCl2；0…

DETECTION OF MINIMAL RESIDUAL DISEASE OF LEUKEMIA BY THE USE OF BONE MARROW SMEARS

In this article, a improved PCR method of detection of minimal residual disease (MRD) was reported, in which, the DNA templates for PCR amplification were extracted from bone marrow smears. The course of DNA extraction was relatively simple and time-saving. Of 60 cases, adequate DNA was obtained in 57 cases. The DNA from 2 to 3 slides could be used for 3 to 4 PCR amplifications. With this method, we detected MRD in 9 children with acute lymphoblastic leukemia after therapy. 4 out of the 9 patients were MRD-positive, and 2 MRD-positive patients relapsed soon after detection, otherwise, the 5 MRD-negative patients didn't relapse. We suggest that this improved MRD-PCR method could be widelyused in clinical practice.