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Metabolomic Study of Polygonatum odoratum Polysaccharide on Hyperthyroidism Yin-deficiency Rats based on GC-MS Metabonomics

??OBJECTIVE To evaluate the effect of Polygonatum odoratum polysaccharide(POP) on hyperthyroidism Yin-deficiency rats based on GC-MS metabolomics and explore its mechanism. METHODS SD rats were randomly divided into control, model and POP groups. The hyperthyroidism Yin-deficiency rats were treated by intraperitoneal injection of T3 for a week. The content of T3??T4 in serum and cAMP??cGMP in plasma was determined. A GC-MS based metabolic profiling method was employed to analyze the distinctive metabolic patterns of serum in control, model and POP. Multivariate statistical analysis was applied for multivariate analysis to find potential biomarkers of Yin-deficiency by SIMCA-P 12.0 software. RESULTS Compared with the model group, polygonatum polysaccharide can significantly reduce the content of T3, T4, cAMP, the ratio of cAMP/cGMP and increase the content of cGMP, indicating it has certain Yin-nourishing action. The PCA score plot showed metabolic profiling of control, model and POP had significant differences. Nine potential biomarkers were identified by PLS-DA and variable importance for project values(VIP) based on the metabolomics. CONCLUSION The endogenous metabolites were changed by POP intervention significantly in Yin-deficiency rats, and the preventive role may be related to the synergism of glucose and lipid metabolism, amino acid metabolism, energy metabolism with the multi-targets, which provides a basis for further study of the mechanism of POP treatment of Yin-deficiency.