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Effect of Curcumol on the Apoptosis Inhibition of TRAF2 and XBP1 Controled by IRE1 in Rats with Obstructive Nephropathy

??OBJECTIVE To investigate the effect of curcumol on the apoptosis inhibition of TRAF2 and XBP1 controled by IRE1 in rats with obstructive nephropathy. METHODS Rats were randomly divided into sham control group, model control group and enalapril group as well as high, medium and low formononetin groups. Animal model of RIF was established by unilateral ureteral obstruction (UUO). The rats were sacrificed at 14 d after UUO, and blood samples were collected. Levels of serum creatinine (Scr) and blood urea nitrogen (BUN) were examined. Renal tubular damage index was determined by H&E staining. The area of RIF was determined by Masson staining. Expressions of GRP78,IRE1,p-IRE1,TRAF2 and XBP1 in kidney were determined by Western blotting analysis. Apoptosis of tubular epithelial cells was detected by TUNEL assay. RESULTS Levels of Scr and BUN, tubulointerstitial injury index, RIF and apoptotic index as well as expressions of GRP78,IRE1,p-IRE1,TRAF2 and XBP1 were different between the model control and treatment groups (P<0.05, P<0.01). Compared with the enalapril group, tubulointerstitial injury index and RIF as well as the levels of Scr and BUN were decreased (P < 0.05) in the high curcumol group. CONCLUSION The curcumol can block the apoptosis of renal tubular epithelial cells through interfering IRE1-XBP1 signaling pathway by inhibiting TRAF2 protein expression mediated by IRE1 phosphorylation. Ultimately, development of RIF was postponed.