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Mycorrhizal Cultivation of Dendrobium officinale

??OBJECTIVE To provide the scientific basis for the application of mycorrhizal fungi in Dendrobium officinale artificial cultivation. METHODS The dominant strain was screened in a planting base by using biomass as the index. The effects of initial inoculums and supplementary inoculation on the growth and the chemical composition of D. officinale were further investigated. RESULTS Mycorrhizal fungi M2(Mycena sp.) was found to be the advantageous strain. After 16 months of growth, the stem dry weight(SDW) and plant dry weight(PDW) of the three M2-treatment groups were significantly higher than those of CK, and the SDW of group M(M2 initial inoculums of 1.0 g??clump^-1) was the highest, which increased by 39.2% and 11.1% compared with CK and group L(M2 initial inoculums of 1.0 g??clump^-1)(P<0.05), respectively. Furthermore, M2 had no effect on the chemical properties of stem polysaccharide, and could improve the contents of naringenin and bibenzyls in the stems. The supplementary inoculation of M2 enhanced the plant-growth-promoting effects of M2. The SDW of the supplementary group increased by 18.0%(P<0.05) compared with the non-supplementary group, while supplementary inoculation had a certain effect on the chemical properties of polysaccharide. CONCLUSION Mycorrhizal fungus M2 has good application prospects for D. officinale artificial cultivation.