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Effect of Ganoderma Spore Oil on Behavior in Depression Mice and Its Neurophysiology Mechanisms

??OBJECTIVE To study the effects of ganoderma spore oil(GSO) on behavior of the mice with chronic unpredictable mild stress (CUMS) and its possible neurophysiology mechanisms. METHODS Thirteen different kinds of chronic unpredictable mild stress were given to the male BALB/C mice for establishing the mouse model of depression. The mice were treated with GSO at 3 doses (850, 283, 141.5 mg??kg^{- 1}??d^-1) or vehicle (oil) or fluoxetine (10 mg??kg^{- 1}??d^-1)] by oral administration from the 3rd week. After 2 weeks administration, the mice was evaluated by behavioral tests, and the contents of hippocampal glutamate (GLU) and ??-amino butyric acid (GABA) were analyzed by reversed phase high performance liquid chromatography (RP-HPLC). The contents of hippocampal brain derived neurotrophic factor (BDNF) were measured by ELISA kit. RESULTS Compared with model group, GSO increased the body weight, sucrose preference rate and open field test score, shortened the immobility time in the tails suspension test and forced swimming test in the depression mice (P<0.05 or P<0.01). Meanwhile, GSO significantly decreased the contents of GLU (P < 0.01 ) and increased the contents of BDNF(P<0.01), and the contents of GABA did not changes (P>0.05) in the hippocampus. CONCLUSION GSO shows obvious anti-depressant effect on depressant model mice. The antidepressant effect of GSO may be related to decreasing GLU contents and increasing BDNF contents.