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| ???????-TiO2??????????????????????о? | |
| 引用本文： | ?????,????,????,????.???????-TiO2??????????????????????о?[J].中国药学杂志,2018,53(14):1198-1202. |
| 作者姓名： | ????? ???? ???? ???? |
| 作者单位： | 1. ?й?????,???? 100022; 2. ?????????????????, ???? 310009; 3. ?????????????(????)????????,?? ??? 317000; 4. ?????,???? 310058 |
| 摘 要： | |
| 关 键 词： | ??????? TiO2?????? ?????? ???? ???? ???? |
Overcoming Cancer Multidrug Resistance by Doxorubicin-TiO2 Nanoparticles |
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| LI Tian-ao,YANG Jian-miao,XU Dong-hang,GAO Jian-qing.Overcoming Cancer Multidrug Resistance by Doxorubicin-TiO2 Nanoparticles[J].Chinese Pharmaceutical Journal,2018,53(14):1198-1202. | |
| Authors: | LI Tian-ao YANG Jian-miao XU Dong-hang GAO Jian-qing |
| Institution: | 1. Chinese Pharmaceutical Association, Beijing 100022, China; 2. Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; 3. Taizhou Hospital of Zhejiang Province, Taizhou Enze Medical Center(Group), Zhejiang Taizhou 317000, China; 4. Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China |
| Abstract: | ??OBJECTIVE To investigate the effect of TiO2 nanoparticles on overcoming cancer multidrug resistance(MDR). METHODS Doxorubicin-TiO2 nanoparticles(DTN) were prepared, the K562/DOX cells were chosen as the model cells. And doxorubicin solution(F-DOX) and doxorubicin liposomes(DOX-L) were also prepared as the control. The MTT assay were measured, and the amount of doxorubicin in the K562/DOX cells at different time were determined by HPLC. The P-gp expression were detected by flow cytometry.RESULTS The MTT assay shows that IC50 of group DTN were lower than that of group F-DOX. The uptake test shows that amount of doxorubicin in K562/DOX cells of group DTN was 1.23 times of group DOX-L when in 4 h, and the efflux test shows that amount of doxorubicin in K562/DOX cells was 1.18 times of group DOX-L. The flow cytometry result revealed that the effect of TiO2 nanoparticles on overcoming MDR maybe through down-regulating the expression of P-gp in K562/DOX cells.CONCLUSION The TiO2 nanoparticles are a new inorganic materials-based nanoparticles which promising approach to overcome MDR. |
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