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| 引用本文： | ???,???,???,Ф??,?????,???,??????,?ζ??.??????????????????????????????????????[J].中国药学杂志,2017,52(7):548-552. |
| 作者姓名： | ??? ??? ??? Ф?? ????? ??? ?????? ?ζ?? |
| 作者单位： | 1. ?????????????????????????????о???,??? 410208; 2. ???????????,???????????????????????????????о?????,????ж?????????????????????????, ??? 410208 |
| 摘 要： | |
| 关 键 词： | ?????????? ??δ??? ??????? ??????? |
Immunomodulatory Effect of Dendrobium officinale Polysaccharide on Macrophage RAW264.7 Induced by LPS |
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| HUANG Jie,ZHANG Ying,YAN Jing,XIAO Rong,XIA Bo-hou,WU Ping,LIN Li-mei,LIAO Duan-fang.Immunomodulatory Effect of Dendrobium officinale Polysaccharide on Macrophage RAW264.7 Induced by LPS[J].Chinese Pharmaceutical Journal,2017,52(7):548-552. | |
| Authors: | HUANG Jie ZHANG Ying YAN Jing XIAO Rong XIA Bo-hou WU Ping LIN Li-mei LIAO Duan-fang |
| Institution: | 1. Stem Cell Laboratory of Regulation and Application, Hunan University of Chinese Medicine, Changsha 410208, China; 2. Hunan University of Chinese Medicine, Hunan Research Center of Engineering Technology for Rapid Test and Removal of Toxic and Harmful Substances in Chinese Medicine, Hunan Engineering Laboratory for Prevention and Control Technology of Toxic Substances in Chinese Medicine, Changsha 410208, China |
| Abstract: | ??OBJECTIVE To study the immunoregulatory effect of Dendrobium officinale polysaccharide (DOP) obtained by step ethanol precipitation on macrophages and explore its action mechanisms. METHODS Step ethanol precipitation method was used to get DOPs of different molecular weights, MTT method was used to detect the protective effect of DOP on macrophages induced by LPS, neutral red method was used to detect the phagocytosis activity of macrophages, ELISA method was used to detect the contents of TNF-??, IL-6, and TGF-??1, and Griess method was used to detect the NO release quantity. RESULTS The yield of DOP was the largest when the ethanol concentration was 75%. DOP could promote the proliferation and phagocytosis activity of RAW264.7 cells, reduce the release of TNF-??, IL-6 and NO, and promote the secretion of TGF-??1 and the proportion of M2 significantly (P<0.01). CONCLUSION Dendrobium officinale polysaccharide has immune regulation effect on RAW264.7 cells, and the mechanism may be related to the promotion of cell phagocytosis activity, inhibition of inflammatory factor release, promotion of TGF-??1 secretion, and increase of the proportion of M2. |
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