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Preliminary Study of Peptide From Periplaneta americana On Antitumor Immunity in L1210-Bearing Mice

??OBJECTIVE To explore the antitumor effect and immunity change of Periplaneta americana C??3 and related synthetic peptide HFDT1 in mice and to provide a base for clinical application of the materials. METHODS Sixty BALB/c mouse tumor model with leukemia L1210 were established and divided into five groups. The mice were treated with Periplaneta americana C??3 high dose, low dose, HFDT1, CTX and normal saline for 10 d, respectively. Twelve mice were as normal control group. The tumor inhibition rates and weight of body were observed in different groups. Then the results were observed including thymus and spleen index, the numbers of immune cells in peripheral blood, the ratio of splenic total lymphocytes, splenic B cell(CD45R⁺)and T cell(CD3⁺), T cell subset(CD3⁺CD4⁺ and CD3⁺CD8⁺ T cell), and the levels of IgG, IgA and IgM in serum through weight, auto-analysis of blood cell, flow cytometry, sandwich-antibody ELISA from thymus, spleen, peripheral blood in the mice. RESULTS The results showed that C??3 and HFDT1 inhibited the growth of mouse L1210 tumor and maintained the mouse weights. They increased the mouse thymus indexes and spleen indexes effectively and the numbers of peripheral blood immune cells. They maintained the spleen lymphocyte and regulated the ratio of T and B lymphocytes, CD3⁺CD4⁺ T cell and CD3⁺CD8⁺T cell. They increased the levels of serum IgG, IgA and IgM. CONCLUSION Periplaneta americana extract C??3 and related synthetic peptide HFDT1 have a strong inhibition effect to tumor. The antitumor effect of C??3 and HFDT1 may be achieved through to improve immune function. They are hoped to become efficient and low toxicity antitumor drugs. Especially HFDT1, it has an extensive application because it can be synthesized by artificial methods.