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Chemical Constituents from Bletilla striata and Their Anti-Tumor Activities

??OBJECTIVE To investigate the chemical constituents from Bletilla striata (Thunb.) Reichb.f and study their anti-tumor activities and effect on progression of cell cycle. METHODS The compounds were isolated by various chromatographic methods including silica gel, ODS, Sephadex LH-20, and so on. Their structures were identified by extensive analysis of spectiroscopic data. The antiproliferative effects of the compounds were evaluated using MTT test, and compound 1 was tested for the effect on the cell cycle of A549 cells. RESULTS Five compounds were isolated from Bletilla striata and identified as 3??-hydroxyoleane-12-en-28-oic acid 3-O-??-L-rhamnopyranosyl-(1??2)-??-D-glucopyranoside(1),2-hydroxysuccinic acid(2),4-hydroxybenzylamine(3),palmitic acid(4),and 4-hydroxybenzoic acid(5). Compound 1 showed antiproliferative activity against the cancer cells and could induce G₀/G₁ phase arrest effectively after 24 h treatment. CONCLUSION Compounds 1-4 are isolated from Bletilla striata for the first time. Compound 1 shows potent inhibitory effect and might produce their action through inducing cell cycle arrest.