��ζ���پ���ȡ��Ը�֬��ʳ�յ�����С���Ӱ��

Effect of Extract from the Stems of Schisandra chinensis on High Fat Diet-Induced Obesity in Mice

??OBJECTIVE To study the effect of extract from the stems of Schisandra chinensis(SCE) on high-fat diet (HFD)induced obesity in mice. METHODS The model of HFD induced obese model of C57BL/6J mice for 8 weeks was established. The contents of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (T-CHO), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol(HDL-C) and hepatic triglyceride (TG), total cholesterol (T-CHO), glutathione (GSH)and malondialdehyde(MDA) were measured. The pathological changes with H&E staining and oil red O was observed for investigating the effect of SCE in the obese mice. In addition, the assay of CYP2E1, 4-HNE using immunofluorescence staining and the expression of iNOS and COX-2 protein with Western blot analyzing was measured to explore the effect of SCE on lipid peroxidation and inflammatory response. RESULTS Compared with the HFD group, low and high dose groups of SCE significantly reduced the serum AST, ALT, TG, T-CHO, LDL-C and hepatic TG, TC and MDA levels, elevated the serum HDL-C and hepatic GSH levels and improved liver tissue steatosis, inflammatory infiltration and accumulation of lipid droplets, oxidative stress and inflammatory response. CONCLUSION SCE may improve lipid metabolism in HFD induced obese mice by inhibiting oxidative stress and inflammatory response.