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The mRNA Expression Change of BKCa Channel in Renal Artery in T2DM Nephropathy Rats

??OBJECTIVE To reveal the mRNA expression change of BK_Ca channel in renal artery in different course in T2DM nephropathy rats.METHODS T2DM nephropathy models were induced by one side nephrotomy, high-fat diet in rats and small doses of streptozotocin through intraperitoneal injection; Real-time PCR were performed to verify mRNA expression. Then body weight, 24 h urinary albumin, blood urea nitrogen(BUN),serum creatinine(Scr), kidney weight are measured. Renal tissue was observed under optical and electron microscope.RESULTS Blood glucose, blood insulin, serum creatinine, urea nitrogen, 24 h urinary albumin, kidney hypertrophy index of T2DM nephropathy rats in 8 weeks and 12 weeks, were significantly higher than that of control group (P<0.05, P<0.01);the rat kidney pathological morphological changes were obvious. Compared with the control group, ?? subunit expression of BK_Ca in the model group at 8 weeks and 12 weeks were obviously unchanged;??₁ subunit expression of BK_Ca were reduced(P<0.05,P<0.01).CONCLUSION The mRNA expression of BK_Ca are reduced in renal artery in different course, and this chang is consistent with the pathological changes degree of kidney in T2DM nephropathy rats.