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Bioavailability and Pharmacokinetics of Nanoparticles of Coix Seed Oil

??OBJECTIVE To delevop a UPLC-MS method for determining the content of olein in rat plasma, and investigate the pharmacokinetics and bioavailability of nanoparticles of coix seed oil in rats. METHODS The rats were divided into three groups randomly. They were administered orally suspension of the raw material of the coix seed oil, Kang lai te soft capsules and nanoparticles of coix seed oil, respectively. Blood samples were collected at 0.083, 0.167, 0.25, 0.5, 0.75, 1, 2, 4, 6, 8, 12, 24, 36, 48, 72, 96, and 120 h and the concentrations of olein in rat plasma were determined by UPLC-MS. The pharmacokinetic parameters were calculated by 3P97 pharmacokinetic program. RESULTS The pharmacokinetic parameters of olein in the the raw material of coix seed oil, KLT soft capsules and nanoparticles of coix seed oil in rat plasma were as follows: ??_max were (5.43??0.45), (7.54??0.44) and (7.30??1.13) mg??L^-1, respectively, with significant difference between the nanoparticles and raw material (P<0.05), while no significant difference between nanoparticles and common oral prepatation(P>0.05); AUC_0-?? were (68.71??5.12), (46.61??3.86), and (178.91??6.26) mg??h??L^-1, respectively, which was the highest for the nanoparticles. The relative bioavailability of olein in the nanoparticles of coix seed oil was 260.38% to the raw material and 383.84% to the common oral prepatation. CONCLUSION The nanoparticles system can increase the bioavailability of coix seed oil and improve its biopharmaceutical property.