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Protection Mechanism of the Main Active Ingredients Combination of Yangyin Tongnao Granules on Cerebral Ischemia/Reperfusion Injury Rats

??OBJECTIVE To explore the protection mechanism of orthogonal compatibility of puerarin(A),ligustrazine(B), astragaloside IV(C) and catalpolon(D),the main active ingredients from yangyin tongnao granules, on SD rats damaged by ischemic reperfusion injury, and select the optimal combination. METHODS SD rats were randomly divided into sham operation group, model group, orthogonal compatibility group and positive control group. The rat middle cerebral artery occlusion (MCAO) model was established. The compatibility of components was arranged by L₉(34) orthogonal design. The symptoms of neurological deficit in rats and the pathological changes in hippocampus were observed; TTC staining was used to detect the cerebral infarction volume. IHC was used to evaluate the expression of NLRP3 protein ischemic brain tissue. RT-PCR was used to detect the expressions of ASC, caspase-1, IL-1, mRNA, IL-18 and MMP-9 in the hippocampal of brain tissue. The RESULTS of orthogonal test were analyzed by range analysis. RESULTS The orthogonal combination groups could effectively improve neurological deficits in cerebral ischemia reperfusion injury, reduce infarction volume, inhibit the expression of NLRP3 inflammasome, decrease the expression of ASC, caspase-1, IL-1, IL-18 and MMP-9. The analysis of test results showed that the combination with ligustrazine 100 mg??kg^-1,puerarin 20 mg??kg^-1, astragaloside ?? 80 mg??kg^-1 and catalpol 20 mg??kg^-1 was the superior one. CONCLUSION The main active ingredients combination of yangyin tongnao granules played a protective mechanism on focal cerebral ischemia reperfusion injury rats. Its mechanism might be related to inhibition of NLRP3 inflammasome,down-regulation of ASC, caspase-1, IL-1, IL-18 and MMP-9.