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Preparation and Anti-Leukemia Efficacy of Daunorubicin-Loaded Microparticles

??OBJECTIVE To construct a novel daunorubicin-loaded microparticles (DNR-MPs)drug delivery system, and study its proliferation inhibiton effect on leukemia cells. METHODS Both DNR-MPs-IDL(DNR microparticles with intracellular drug loading method)and DNR-MPs-EDL (DNR microparticles with extracellular drug loading method)were prepared from HL-60 cells, and the average particle size, Zeta potential and drug loading efficiency of DNR-MPs were measured. The uptake of DNR-MPs by HL-60 cells was analyzed by confocal laser scanning microscopy (CLSM)and flow cytometry (FCM). The inhibition effect of DNR-MPs on the proliferation of HL-60 cells was evaluated by MTT assay. RESULTS There was no significant difference in the average particle size and Zeta potential of the two DNR-MPs. The drug loading efficiency of DNR-MPs-EDL was higher compared with DNR-MPs-IDL. The uptake RESULTS showed that the two DNR-MPs significantly increased the drug uptake compared with free DNR (P<0.05). DNR-MPs-IDL showed a higher drug uptake by the cells than DNR-MPs-EDL did (P<0.05). They also exhibited an enhanced inhibition effect on the proliferation of HL-60 cells compared with DNR (P<0.05). CONCLUSION The different ways of preparation of drug-loaded microparticles can cause varying anti-tumor effect. Microparticles can significantly augment the anti-leukemia efficacy of DNR, indicating a promising drug carrier for the therapy of leukemia.