�˰����������᳦��ϸ��HCT116��ֲ����������������

Inhibitory Effect of Ke-ai-xin Pill on Tumor Growth of Transplanted Colon Cancer Cell Line HCT116 in Nude Mice

??OBJECTIVE To study the inhibitory effect of Ke-ai-xin pill on tumor growth and angiogenesis of transplanted colon cancer cell line HCT116 in nude mice. METHODS To establish the transplant tumor model in nude mice for HCT116. All tumor-bearing nude mice were randomly divided into control group (normal saline), positive group (bevacizumab,5 mg??kg^-1, intraperitioneally), and low-, medium-, high-dose Ke-ai-xin pill-treated groups(200, 400 and 800 mg??kg^{- 1}, intragastrically). The tumor xenografts were harvested and measured for their weights. Immunohistochemistry was used to detecte microvessel density (MVD)and vascular endothelial growth factor(VEGF)expression. RESULTS Ke-ai-xin pill inhibited tumor growth in nude mice in varying degrees. After the administration of each treatment group, TV, RTV, and T/C decreased significantly(P<0.05). SABC assay showed that MVD of Ke-ai-xin pill treatment group was sparse, MVD value decreased from (59.62??18.83) to (55.56??12.63),(46.72??11.57) and (41.35??10.27), Ke-ai-xin pill treatment group can significantly reduce the positive expression of VEGF. the positive expression rate reduced from(78.46??16.53)% to (58.64??14.19)%, (49.27??13.29)% and (43.68??11.71)%. CONCLUSION Ke-ai-xin pill can inhibit tumor growth of transplanted colon cancer cell line HCT116 in nude mice in varying degrees, reduce MVD value and the expression of VEGF in tumor tissue, so its antitumor activity may be related to inhibiting angiogenesis.