lncRNA SNHG30基因敲除通过抑制PHF5A表达影响胶质瘤U251细胞增殖

目的探讨长链非编码RNA(lncRNA)SNHG30基因敲除对胶质瘤U251细胞增殖能力的影响及可能的机制。 方法根据人源SNHG30基因序列特征构建px330-sgRNA质粒并转染U251细胞。采用PCR方法和测序鉴定筛选敲除SNHG30基因的U251细胞株。通过GEPIA2网站分析SNHG30在胶质瘤中的表达情况,CCK-8检测SNHG30敲除对U251细胞增殖的影响,使用CYTOSCAPE软件进行关键基因PHF5A分析并进行qRT-PCR验证。 结果成功获得一株SNHG30基因完全敲除的U251细胞株。SNHG30在胶质瘤组织中高表达,而SNHG30基因敲除U251细胞株的增殖能力和PHF5A的表达量降低。 结论lncRNA SNHG30基因敲除的U251细胞株增殖能力下降,可能与其抑制PHF5A的表达有关。

Effect of lncRNA SNHG30 knockout on proliferation of glioma U251 cells through inhibits PHF5A expression

AimTo investigate the effect of lncRNA SNHG30 gene knockout on the proliferation of glioma U251 cells and its possible mechanism. MethodsAccording to the sequence characteristics of human SNHG30 gene, px330-sgRNA plasmid was constructed and transfected into U251 cells. The SNHG30 knockout U251 cell lines were identified by PCR and sequencing. The expression of SNHG30 in glioma was analyzed by GEPIA2. The effects of SNHG30 disruption on the growth of U251 cells were detected by CCK-8. CYTOSCAPE were used for PHF5A hub genes analysis and the resulting gene was verified through qRT-PCR. ResultsU251 cell line with complete SNHG30 gene knockout was successfully obtained. SNHG30 was highly expressed in glioma tissues, while the proliferation ability SNHG30 knockout U251 cell line and the expression of PHF5A decreased. ConclusionlncRNA SNHG30 gene knockout reduces the proliferation ability of U251 cell line, which may be related to the inhibition of PHF5A expression.