黄芪甲苷对脊髓损伤及脊髓胶质细胞凋亡的影响

目的探讨黄芪甲苷(AS)对脊髓损伤(SCI)及脊髓胶质细胞凋亡的影响。 方法实验分为对照组、SCI组(SCI模型)、AS低剂量组(AS-L组)、AS高剂量组(AS-H组)。比较各组大鼠运动功能、脊髓组织水肿、细胞凋亡、氧化反应情况；蛋白免疫印迹分析各组脊髓组织核因子E2相关因子2(Nrf2)和血红素加氧酶-1(HO-1)水平。 结果与对照组比较,SCI组脊髓组织促凋亡相关的蛋白cleaved Caspase-3和Bax表达升高,出现明显广泛的神经元死亡、胶质细胞凋亡和氧化应激反应,并伴有更严重的功能缺陷。而AS能够呈剂量依赖性逆转上述趋势,且AS-L组、AS-H组脊髓组织Nrf2和HO-1水平高于对照组和SCI组。 结论AS通过调节Nrf2/HO-1信号通路具有抗氧化和抗凋亡作用,进而改善大鼠脊髓损伤,为脊髓损伤的治疗提供新的思路。

Effects of astragaloside IV on spinal cord injury and apoptosis of spinal cord glial cells

AimTo investigate the effects of astragaloside IV (AS) on spinal cord injury and apoptosis of spinal cord glial cells. MethodsThe rats were divided into control group, spinal cord injury (SCI) group (SCI model), AS low-dose group (AS-L group) and AS high-dose group (AS-H group). The motor function, spinal cord tissue edema, cell apoptosis and oxidative stress were compared among the groups. The levels of nuclear factor E2 related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) in spinal cord tissue were analyzed by Western blot. ResultsCompared with the control group, the expression of pro-apoptotic proteins (cleaved Caspase-3 and Bax) in the spinal cord tissue of the SCI group was increased, and there was obvious neuronal death, glial cell apoptosis and oxidative stress, accompanied with more severe functional defects. However, AS reversed the above trend in a dose-dependent manner, and the levels of Nrf2 and HO-1 in the spinal cord tissues of AS-L and AS-H groups were higher than those of the control and SCI groups. ConclusionAS has antioxidant and anti-apoptotic effects by regulating Nrf2/HO-1 signaling pathway, and thus improves spinal cord injury in rats. These results may provid new ideas for the treatment of spinal cord injury.