葡萄籽原花青素对大鼠实验性糖尿病肾病的影响

目的观察葡萄籽原花青素(GSPE)对糖尿病(DM)大鼠早期肾损伤的影响并探讨其作用机理。方法采用四氧嘧啶糖尿病大鼠模型,灌胃给以GSPE 6周后,测定各组大鼠的肾功能指标,肾组织和血清的抗氧化指标及一氧化氮(NO)含量、一氧化氮合酶(NOS)活性。结果GSPE组大鼠的24h尿蛋白、血清尿素氮、血肌酐、肌酐清除率和肾指数低于DM组,肾组织SOD活性高于DM组,MDA含量低于DM对照组(P<0.05)。GSPE低剂量组大鼠的肾脏NO含量、血清及肾脏NOS活性低于DM对照组(P<0.05)。结论GSPE对糖尿病大鼠肾脏具有保护作用,其机制与提高DM大鼠肾脏的抗氧化能力,降低肾脏和血清NO含量、NOS活性有关。

Effects of grape seed proanthocyanidins extracts on experimental diabetic nephropathy in rats

Objective To study the effects of grape seed proanthocyanidins extracts(GSPE) and its mechanism on early renal lesions of diabetic rats.Methods Diabetic rats induced by alloxan were given GSPE intragastrically for 6 weeks,then the antioxidative indexes and NO content,NOS activity in kidney and serum were measured,and the renal function indexes were tested as well.Results Compared with the diabetic group,the urinary protein/24h,levels of blood urea nitrogen(BUN)and serum creatinine(SCr),creatinine clearance rate(CCr)and the ratio of kidney weight/body weight were decreased,the SOD activity in kidney was raised while MDA content was fall in the GSPE group(high dose),and the differences were all significant.The NO content in the kidney and NOS activity in kidney and serum decreased in the GSPE(low dose)group,and there was significant difference when compared with diabetic group(P<0.05).Conclusion GSPE has the effect in protecting kidney of diabetic rats,the mechanism might be related with its action in increasing the renal antioxidative ability,decreasing the content of NO and the activity of NOS in kidney and serum.