白血病患儿巨细胞病毒感染的PCR与抗原检测方法比较

目的 对不同种类标本应用ＰＣＲ检测巨细胞病毒（ｃｙｔｏｍｅｇａｌｏｖｉｒｕｓ，ＣＭＶ）ＤＮＡ，并与外周血白细胞ＣＭＶ抗体检测比较，以了解ＰＣＲ方法在诊断白血病患儿活动性ＣＭＶ感染时的意义。方法 实验组为３１例白血病患儿，对照组为３１例免疫功能正常儿童，两组儿童的年龄、性别无差异。应用ＰＣＲ方法检测血清、尿液和脑脊液ＣＭＶ ＤＮＡ，间接免疫荧光方法检测外周血白细胞ＣＭＶ抗原，ＥＬＩＳＡ方法检测血清抗

Cytomegalovirus infection in children with leukemia: PCR assay for detecting CMV-DNA and CMV antigenemia assay

Objective To evaluate the incidence and clinical course of CMV infection in leukemia children, and to compare PCR assay for detecting CMV DNA and antigenemia assay for detecting CMV antigen in peripheral blood leukocytes. Methods Totally 31 leukemia children and 31 immunocompetent children as a control group were involved in the study. PCR assay was used for detecting CMV DNA in serum, urine and cerebrospinal fluid, indirect immunofluorescence assay for detecting CMV antigen in peripheral blood leukocytes, and ELISA for detecting anti CMV IgG. Results Twenty seven out of thirty one leukemia children had anti CMV IgG before chemotherapy. The rest 4 patients were found seroconverted after chemotherapy. CMV infection rate was 100% in leukemia children compared with 83 9%(26/31) in control group. There was no difference in CMV infection rate between two groups ( P =1). Active CMV infection was diagnosed in 29 1%(9/31) leukemia children while none in control group. Active CMV infection rate showed significant difference between two groups ( P =0 002). The sensitivity and specificity of PCR for detecting CMV DNA in urine, serum and cerebrospinal fluid versus the antigenemia assay were 88 9% 100% and 68 2% 81 8%, respectively. Conclusions Activation of CMV infection in leukemia children are rather common. PCR assay on urine, serum and cerebrospinal fluid can be useful for diagnosing CMV infection for its high sensitivity, but it should be noticed that the active CMV infection and the latent CMV infection cannot be easily differentiated.