半嵌套式RT-PCR法检测肾病患者尿沉渣中WT1基因表达研究

目的探讨尿沉渣中WT1基因表达在肾脏病检测中的意义。方法采用半嵌套式RT—PCR方法检测健康人，慢性肾小球肾炎(CGN)，风湿性疾病以及糖尿病肾病等进行性肾损害患者尿中WT1基因表达情况。结果在尿蛋白阳性的CGN和DM患者，尿沉渣中WT1表达阳性率分别为46.7％(14／30)和44．4％(16／36)；在尿蛋白阴性的DM患者，尿沉渣中WT1基因表达阳性率5.95%(5／84)；在风湿性疾病患者，尿沉渣中WT1基因表达阳性率为16．0％(4／25)；WT1基因表达与蛋白尿阳性程度，血尿，肾功能及临床病程无关；正常人，多囊肾以及慢性膀胱炎患者，尿沉渣中WT1基因表达均为阴性。结论尿中WT1基因检测对进行性肾脏损害的早期诊断具有十分重要的意义。

The Study of WT1 Gene Expression in the Urine from Patients with Renal Diseases by Semi-nested PCR

Objective To investigate the clinical significance of WT1 gene expression in the urine. Methods The expression of WT1 gene in the urine of patients with chronic glomerular nephritis(CGN), rheumatic diseases,diabetes mellitus(DM) and healthy subjects was detected by semi-nested PCR. Results The positive rates of WT1 gene expression in CGN with proteinuria,DM with proteinuria, DM without proteinuria and rheumatic diseases were 46.7% (14/30),44.4% (16/36),5.95% (5/84) and 16%(4/25), respectively. WT1 expression in the urine was not related to the degree of proteinuria, hematuria, renal function or clinical course of diseases. WT1 expression in the urine of the healthy subjects or cystitis patients was negative. Conclusion The measurement of WT1 mRNA in the urine could play an important role in early diagnosis of progressive renal damage.