Effects of 5-aminolevulinic acid photodynamic therapy on TLRs in acne lesions and keratinocytes co-cultured with P. acnes |
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| Affiliation: | 1. Department of Dermatology, Huashan Hospital, Fudan University, Shanghai 200040, PR China;2. University of Colorado Denver Cancer Center, Aurora, CO, USA;3. MOE Key Laboratory of OptoElectronic Science and Technology for Medicine, Fujian Normal University, Fuzhou 350007, PR China;1. Professor Rubem David Azulay Dermatology Institute, Santa Casa da Misericórdia do Rio de Janeiro, Rio de Janeiro, Brazil;2. Ipanema Federal Hospital, Internal Medicine and Dermatology Departments, Rio de Janeiro, Brazil;3. Dermatology Unit, Second University of Naples, Naples, Italy;4. Federal University of Rio de Janeiro, Pathology Department, Rio de Janeiro, Brazil;1. Institute of Molecular Animal Breeding and Biotechnology Gene Center, LMU Munich, Munich, Germany;2. Laboratory of Cutaneous Physiopathology and Integrated Center of Metabolomics Research, San Gallicano Dermatologic Institute, IRCCS, Rome, Italy;3. Departments of Dermatology, Venereology, Allergology and Immunology, Dessau Medical Center, Dessau, Germany;1. Institute of Molecular Animal Breeding and Biotechnology Gene Center, LMU Munich, Munich, Germany;1. Drug Development Research Laboratories, Kyoto R&D Center, Maruho Co., Ltd., Japan;2. CMC Research Laboratories, Kyoto R&D Center, Maruho Co., Ltd., Japan;3. Department of Microbiology and Infection Control Science, Kyoto Pharmaceutical University, Japan;4. Clinical Trial Testing Department, LSI Medience Co., Japan;5. Department of Dermatology, Tokyo Women''s Medical University, Japan |
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| Abstract: | ObjectiveTo investigate the effect of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) on the expression of Toll like receptors (TLRs) in human keratinocytes and its role in acne treatment.MethodsTLR2 and TLR4 expression in acne lesions before and after ALA-PDT were examined by immunohistochemical assay. Primary keratinocytes were obtained from acne lesions, co-cultured with P. acnes and then treated with ALA-PDT using red or blue LED. Cytokines production were examined by ELISA, TLR2 and TLR4 gene expression by real-time PCR, and TLR2 and TLR4 protein expression by Western-blot assay.ResultsThe overexpression of TLR2 and TLR4 in acne lesion were detected, which became negative or weaker after ALA-PDT. The infection of P. acnes in keratinocytes could significantly increase the levels of early inflammatory cytokines (e.g. IL-1α, TNF-α and IL-8) (P < 0.05). Such responses could be inhibited by ALA-PDT. P. acnes infection could also significantly increase TLR2 and TLR4 expressions in keratinocytes (P < 0.05), which could be down-regulated by ALA-PDT.ConclusionsALA-PDT could inhibit innate immune responses in keratinocytes treated with P. acnes via TLRs pathways. |
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| Keywords: | 5-Aminolevulinic acid Photodynamic therapy Keratinocytes Toll like receptors Cytokines |
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