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| 小檗碱对白介素-6诱导胰岛素抵抗 3T3-L1脂肪细胞脂联素表达的影响 | |
| 引用本文： | 章常华,李冰涛,屈飞,魏学鑫,余日跃,徐国良. 小檗碱对白介素-6诱导胰岛素抵抗 3T3-L1脂肪细胞脂联素表达的影响[J]. 中国实验方剂学杂志, 2012, 18(23): 164-167 |
| 作者姓名： | 章常华 李冰涛 屈飞 魏学鑫 余日跃 徐国良 |
| 作者单位： | 1. 江西中医学院药学院,南昌 330004;中山大学中山医学院药理学教研室,广州 510080 2. 江西中医学院现代中药制剂教育部重点实验室,南昌,330004 3. 江西中医学院药学院,南昌,330004 |
| 基金项目： | 国家科技部973计划项目(2010CB530603) ;国家自然科学基金项目(81260660) |
| 摘 要： | 目的:观察小檗碱对白介素-6(IL-6)诱导胰岛素抵抗的3T3-L1脂肪细胞脂联素表达的影响。方法:选用IL-6诱导3T3-L1脂肪细胞胰岛素抵抗(IR)模型。以20μg·L-1IL-6培养48 h,将3T3-L1脂肪细胞随机分为正常对照组、模型组、吡格列酮组(50μmol.L-1)和小檗碱高、中、低剂量组(10,20,50μmol.L-1),以葡萄糖氧化酶法测定葡萄糖消耗量,观察小檗碱对脂肪细胞葡萄糖摄取的影响,鉴定IR模型;采用实时荧光定量PCR技术测定脂肪细胞脂联素基因mRNA水平。结果:模型组葡萄糖消耗量及脂联素基因表达水平与正常对照组比较,均显著降低(P<0.05);小檗碱高、中、低剂量组及吡格列酮组均能显著增加葡萄糖消耗量及脂联素基因表达水平(P<0.05)。结论:小檗碱可增加白介素-6诱导胰岛素抵抗的3T3-L1脂肪细胞脂联素基因mRNA的表达,改善胰岛素抵抗状态。 |
| 关 键 词： | 小檗碱 胰岛素抵抗 白介素-6 脂联素 3T3-L1细胞 |
| 收稿时间： | 2012-05-27 |
Effect of Berberine on Gene Expression of Adiponectin by IL-6 Induced Insulin Resistance in 3T3-L1 Adipocyte |
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| ZHANG Chang-hu,LI Bing-tao,QU Fei,WEI Xue-xin,YU Ri-yue and XU Guo-liang. Effect of Berberine on Gene Expression of Adiponectin by IL-6 Induced Insulin Resistance in 3T3-L1 Adipocyte[J]. China Journal of Experimental Traditional Medical Formulae, 2012, 18(23): 164-167 | |
| Authors: | ZHANG Chang-hu LI Bing-tao QU Fei WEI Xue-xin YU Ri-yue XU Guo-liang |
| Affiliation: | Department of Pharmacology, College of Pharmacy, Jiangxi University of Traditional Chinese Medicine (TCM), Nanchang 330004, China;Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, China;Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of TCM, Nanchang 330004, China;Department of Pharmacology, College of Pharmacy, Jiangxi University of Traditional Chinese Medicine (TCM), Nanchang 330004, China;Department of Pharmacology, College of Pharmacy, Jiangxi University of Traditional Chinese Medicine (TCM), Nanchang 330004, China;Department of Pharmacology, College of Pharmacy, Jiangxi University of Traditional Chinese Medicine (TCM), Nanchang 330004, China;Key Laboratory of Modern Preparation of TCM, Ministry of Education, Jiangxi University of TCM, Nanchang 330004, China |
| Abstract: | |
| Keywords: | berberine insulin resistance interleukin-6 adiponectin 3T3-L1 cells |
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