| 高效液相色谱梯度洗脱法测定大鼠胆汁中9-硝基喜树碱含量 |
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| 引用本文： | 李柯,徐静华,薛开会,钟大放.高效液相色谱梯度洗脱法测定大鼠胆汁中9-硝基喜树碱含量[J].沈阳药科大学学报,2002,19(2):101-104. |
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| 作者姓名： | 李柯 徐静华 薛开会 钟大放 |
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| 作者单位： | 1. 沈阳药科大学药物代谢与药物动力学实验室,辽宁,沈阳,110016
2. 沈阳药科大学药学院,辽宁,沈阳,110016 |
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| 基金项目： | 国家自然科学基金资助项目 ( 39930 1 80 ) |
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| 摘 要： | 目的建立大鼠胆汁中 9 硝基喜树碱的高效液相色谱梯度洗脱方法。方法胆汁样品经乙腈 水 ( 1∶1 )稀释后 ,直接进样。检测波长为 370nm ,采用HypersilBDSC18柱 ( 5 μm,1 5 0mm× 4 6mmI D )分离 ,使用以乙腈 2 %甲酸水溶液为流动相的二元梯度洗脱方法。结果线性范围为 0 5～2 5 μg/mL,定量限为 0 5 μg/mL。结论本法操作简便 ,成功应用于胆汁中 9 硝基喜树碱的含量的测定及胆汁中 9 硝基喜树碱代谢物的初步研究。
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| 关 键 词： | 9-硝基喜树碱 高效液相色谱法 梯度洗脱 胆汁 |
| 文章编号： | 1006-2858(2002)02-0101-04 |
| 修稿时间： | 2001年7月6日 |
Determination of 9-nitrocamptothecin in the rat′s bile by HPLC using the gradient elution |
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| LI Ke ,XU Jing hua ,XUE Kai hui ,ZHONG Da fang.Determination of 9-nitrocamptothecin in the rat′s bile by HPLC using the gradient elution[J].Journal of Shenyang Pharmaceutical University,2002,19(2):101-104. |
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| Authors: | LI Ke XU Jing hua XUE Kai hui ZHONG Da fang |
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| Institution: | LI Ke 1,XU Jing hua 2,XUE Kai hui 1,ZHONG Da fang 1 |
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| Abstract: | Objective To develop a novel HPLC UV method for determination of 9 nitrocamptothecin (9 NC) in the rat′s bile. Method The samples were separated on a Hypersil BDS C 18 column after being diluted by acetonitrile water (1∶1). The mobile phase consisted of acetonitrile and water, and a gradient elution program was applied. The UV detector was set at 370 nm. Result The liner calibration curves were obtained in the concentration range of 0 5 ～ 25 μg/mL . The limit of quantification was 0 5 μg/mL . Conclusion The method was simple and successfully applied to determining the concentrations of 9 NC in the bile samples, and it also offered a way to the preliminary study on the metabolites of 9 NC in the rat′s bile. |
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| Keywords: | nitrocamptothecin HPLC gradient elution bile |
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