Structure of T cell antigen receptor beta chain in synovial fluid cells from patients with rheumatoid arthritis |
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Authors: | E C Keystone M Minden R Klock L Poplonski J Zalcberg T Takadera T W Mak |
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Affiliation: | Department of Medicine, Ontario Cancer Institute, Princess Margaret Hospital, Toronto, Canada. |
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Abstract: | We attempted to identify a clonal proliferation of T cells from synovial fluid samples from patients with rheumatoid arthritis, using techniques of restriction fragment length polymorphism. We used probes for the beta chain of the T cell receptor to analyze restriction fragments prepared from the genomic DNA of synovial fluid mononuclear cells from 10 patients and synovial fluid T cell preparations from 5 additional patients. The results demonstrated unarranged (germline) T cell receptor gene fragments of DNA in all cell preparations, indicating the lack of clonality of rheumatoid arthritis synovial fluid T cells. |
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