乙型肝炎病毒表面抗原阴性271例隐匿性感染的研究

目的了解在乙型肝炎表面抗原（HBsAg）阴性人群中隐匿性感染情况。方法采用实时荧光定量聚合酶链反应检测271例重庆黔江区HBsAg阴性住院患者血清中HBV-DNA含量。结果271例HBsAg阴性人群血清HBV—DNA阳性率为10．7％，HBV-DNA含量均低于10^3 copy／mL。HBV—DNA检出率与性别、年龄无关。在HBV-DNA阳性人群中抗-HBc抗体出现率较高，抗-HBs阳性或乙型肝炎病毒标志物全阴性也可检出HBV-DNA。结论血清HBsAg阴性者存在一定比例的隐匿性感染，对不明原因的肝损害、输血、器官移植等应结合灵敏度高的HBV-DNA检测结果再作判断。

Analysis of occult hepatitis B virus infection in 271 cases with serum HBsAg negative

Objective To investigate the prevalence of occult hepatitis B virus infection in population with serum hepatitis B surface antigen negative. Methods Real time fluorescent quantitative polymerase chain reaction （RT- PCR） was applied to measuring the serum content of HBV-DNA in 271 inpatients with serum HBsAg negative. Resuits The positive rate of serum HBV-DNA was 10.7% in 271 inpatients with serum HBsAg negative. The content of HBV-DNA was below 103 copies/mL, and the detection rate of HBV-DNA wasnlt associated with age and gender. Anti-HBc antibody was more common in HBV-DNA positive subjects and HBV-DNA was still detected in the samples of anti-HBs antibody positive or negative for any serological indicator of HBV. Conclusion Occult hepatitis B virus infection occurs in some hepatitis B surface antigen negative individuals, and it is necessary to introduce highly sensitive HBV-DNA detection method in case of cryptogenic hepatic lesion, blood transfusion, and organ transplantation.