| 鳖甲及其混伪品的DNA分子鉴定 |
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| 引用本文： | 杜 鹤,崔丽娜,张 辉,姚 辉,宋经元,陈士林. 鳖甲及其混伪品的DNA分子鉴定[J]. 世界科学技术-中医药现代化, 2011, 13(2): 429-434 |
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| 作者姓名： | 杜 鹤 崔丽娜 张 辉 姚 辉 宋经元 陈士林 |
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| 作者单位： | 杜鹤 (长春中医药大学中医药与生物工程研发中心,长春,130117) ； 崔丽娜 (长春中医药大学中医药与生物工程研发中心,长春,130117) ； 张辉 (长春中医药大学中医药与生物工程研发中心,长春,130117) ； 姚辉 (中国医学科学院,药用植物研究所,北京,100193) ； 宋经元 (北京协和医学院,药用植物研究所,北京,100193) ； 陈士林 (中国医学科学院,药用植物研究所,北京,100193) ； |
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| 基金项目： | 吉林省财政厅项目:药用动物DNA务形码鉴定技术研究,负责人:张辉 |
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| 摘 要： | 目的:探讨快速、准确鉴定鳖甲及其混伪品的方法.方法:本文对中华鳖及其混伪品的COI序列用MEGA 4.0等软件进行遗传距离等分析,并构建中华鳖及其混伪品的NJ树.结果:中华鳖种内COI序列变异很小,种间存在较多的变异位点,种间的遗传距离显著大于种内的遗传距离.由所构建的系统聚类树图可以看出,同属聚在一起,且各物种又形成...
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| 关 键 词： | 鳖甲 COI 鉴定 DNA条形码 |
| 收稿时间： | 2011-03-04 |
| 修稿时间： | 2011-04-06 |
Molecular Identification of Trionyx Sinensis and Its Adulterants Based on COI Barcode Sequence |
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| Du He,Cui Lin,Zhang Hui,Yao Hui,Song Jingyuan and Chen Shilin. Molecular Identification of Trionyx Sinensis and Its Adulterants Based on COI Barcode Sequence[J]. World Science and Technology—Modernization of Traditional Chinese Medicine and Materia Medica, 2011, 13(2): 429-434 |
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| Authors: | Du He Cui Lin Zhang Hui Yao Hui Song Jingyuan Chen Shilin |
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| Affiliation: | Development Center of Traditional Chinese Medicine and Bioengineering,Changchun University of Chinese Medicine,Changchun 130117,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100193,China |
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| Abstract: | This study aimed to quickly and accurately differentiate Trionyx sinensis from its adulterants by using COI barcode.Genetic distances of COI sequences of Trionyx sinensis and its adulterants were analyzed by MEGA 4.0 and the NJ tree was constructed.The results showed that the intra-specific variation of T.sinensis exhibited low and the inter-specific divergence exhibited high.The inter-specific divergences were higher than the intra-specific variations.The cluster dendrogram showed that the same genus is together,the different species separated well,and basically support higher rate.It is concluded that as a DNA barcode,COI sequence is able to distinguish the Trionyx sinensis from its adulterants accurately. |
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