| 钙调蛋白拮抗剂苄基异喹啉类化合物对培养的成纤维细胞的作用机理研究 |
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| 引用本文： | 刘为民,赵轼轩,刘杰文. 钙调蛋白拮抗剂苄基异喹啉类化合物对培养的成纤维细胞的作用机理研究[J]. 中国药理学通报, 1992, 0(5) |
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| 作者姓名： | 刘为民 赵轼轩 刘杰文 |
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| 作者单位： | 中国医学科学院血研所 天津300020(刘为民，赵轼轩)，中国医学科学院血研所 天津300020(刘杰文) |
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| 摘 要： | 本文将牛血清白蛋白(BSA)与钙调蛋白(Calmodulin,CaM)交联作为抗原,制备抗体,运用酶联免疫吸附法(ELISA)建立了测定CaM含量的标准曲线,通过EL ISA和流式细胞光度计观察了CaM拮抗剂0—(1—ethoxyl—bUtyl)berbarmine(EBB)对培养的成纤维细胞的CaM和DNA的影响,探讨了EBB的作用机理。
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| 关 键 词： | 成纤维细胞 CaM EBB ELISA |
Inhibitory effects of calmodulin antagonist EBB on cultured fibroblast |
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| LIU Wei -Min ZHAO Shi - Xuan LIU Jie-Wen. Inhibitory effects of calmodulin antagonist EBB on cultured fibroblast[J]. Chinese Pharmacological Bulletin, 1992, 0(5) |
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| Authors: | LIU Wei -Min ZHAO Shi - Xuan LIU Jie-Wen |
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| Abstract: | Inhibitory effects of the O - ( 1 - ethoxvl - butyl ) berbarmine (EBB) on cultured fibroblast was studied by observating calmodulin (CaM ) content of cultured fibroblast with ELISA and DNA content at each phase of cell cycle with flow - cvtometerv. The CaM con-tent in the test group . compared to control . decreased markedly and DNA content increased significantly in the G0+Gi phase but reduced in the S phase. These results suggested that inhibitory mechanism of EBB on fibroblast proliferation may be closely related to CaM decrease in cells. |
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| Keywords: | CaM: EBB: DNA ELISA : fibroblast |
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