Establishment of the mimetic aging effect in mice caused by D-galactose |
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Authors: | Ho Shih-Ching Liu Jue-Hao Wu ReY-Yih |
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Institution: | (1) Traditional Chinese Medicine Division, Development Center for Biotechnology, 81 Chang Hsing Street, Taipei, Taiwan, R.O.C. |
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Abstract: | We successfully established an oxidant damageof mimetic aging model using mice induced byD-galactose, and the mimetic aging model isrelative to free radical and the accumulationof waste substances in metabolism. The animalswere divided into 3 groups: (1)phosphate-buffered saline (PBS); (2) 1%D-galactose; (3) 5% D-galactose bysubcutaneous injection every day. After 45days, mice treated with D-galactose showed asignificant increase in the malondialdehyde(MDA), total antioxidant status (TAS) and adecrease in superoxide dismutase (SOD) in the blood comparedwith the PBS group. In the brain, theD-galactose treated mice exhibited a higherlevel MDA and a lower level SOD activity.In the liver, only the 5% D-galactose groupindicated a significant increase in MDA. By reference to theoxidative biomarkers in blood, brain and liver,we have confirmed the reliability of themimetic aging model. |
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Keywords: | D-dalactose free radical malondialdehyde mimetic aging model superoxide dismutase total antioxidant status |
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