| 维甲酸对宫颈癌耐药细胞株分化及凋亡影响的研究 |
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| 引用本文： | 胡海燕,古衍.维甲酸对宫颈癌耐药细胞株分化及凋亡影响的研究[J].齐齐哈尔医学院学报,2011,32(12):1881-1883. |
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| 作者姓名： | 胡海燕 古衍 |
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| 作者单位： | 南方医科大学附属深圳妇幼保健院,广东 |
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| 摘 要： | 目的研究全方式维甲酸（ATRA）对宫颈癌耐药细胞株HeLa/MMC诱导分化及凋亡的影响。方法采用MTT法检测HeLa/MMC细胞生长速度;集落形成实验观察ATRA对HeLa/MMC细胞集落形成能力的影响;在透射电镜下观察ATRA对HeLa/MMC超微结构的影响;TUNEL法检测HeLa/MMC细胞实验组与对照组细胞凋亡率;半定量RT-PCR检测凋亡相关基因bax的表达。结果通过实验观察到ATRA联合MMC能明显抑制HeLa/MMC细胞的增殖;ATRA作用后的HeLa/MMC细胞出现成熟细胞的特征、细胞集落形成能力降低;ATRA能促进细胞凋亡,上调HeLa/MMC细胞bax基因的表达。结论表明ATRA能有效的诱导宫颈癌耐药细胞株HeLa/MMC分化,并促进其凋亡。诱导凋亡相关基因bax基因的表达上调是ATRA作用的分子机制之一。
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| 关 键 词： | 宫颈肿瘤 维甲酸 细胞分化 细胞凋亡 |
Effect of all-trans retinoic acid on differentiation and apoptosis of drug resistance cervical carcinoma cell |
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| HU Hai-yan,et al..Effect of all-trans retinoic acid on differentiation and apoptosis of drug resistance cervical carcinoma cell[J].Journal of Qiqihar Medical College,2011,32(12):1881-1883. |
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| Authors: | HU Hai-yan |
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| Institution: | HU Hai-yan,et al.(The Affiliated Shenzhen Maternity and Child Healthcare Hospital of Nanfang Medical University,Shenzhen,Guangdong 518000,the P.R.China.) |
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| Abstract: | Objective To investigate the effect of all-trans retinoic acid(ATRA) on differentiation and apoptosis of HeLa/MMC cells.Methods The HeLa/MMC cell proliferation was studied with MTT assay after treatment of ATRA.The efficiency of colony formation was studied by colony formation test.The microstructure of the cells was observed by electron microscope.The apoptosis efficiency was studied by TUNEL.The changes of bax mRNA levels were determined by semiguantiative RT-PCR.Results ATRA could inhibit the proliferati... |
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| Keywords: | Cervical tumor Retinoic acid Cell differentiation Apoptosis |
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