耐药性癫痫患者脑组织中腺苷酸激酶2蛋白的表达及临床意义

目的 研究耐药性癫痫患者脑组织中腺苷酸激酶2(AK2)蛋白的表达,探讨其在耐药性癫痫发生及发展中的作用.方法 用基因芯片、免疫组织化学、免疫荧光检测AK2在40例耐药性癫痫患者术后脑组织标本中的表达,按照配对设计原则与性别相同、年龄近似的20例对照组进行比较.结果 免疫组化结果 显示在对照组AK2蛋白未见表达,而在耐药性癫痫患者脑组织中AK2有明显的棕黄色阳性表达.免疫荧光结果 显示对照组AK2蛋白未见表达,在耐药性癫痫组表达明显,主要分布于细胞胞体.结论 耐药性癫痫患者脑组织中,异常表达的AK2可能参与了耐药性癫痫的形成并有可能成为是耐药性癫痫存在的一个重要标志.

Expression of adenkylate kinsaes 2 in the brains of patients with refractory temporal epilepsy

Objective To study the protein expression of adenkylate kinsaes (AK)-2 in the brain tissues of patients with drug-refractory temporal epilepsy and the clinical value thereof. Methods Specimens of brain from 40 patients with refractory temporal epilepsy, and 20 sex- and age-matched persons with brain trauma. Immunohistochemistry and immunofluoreseence examination were used to test the levels of AK2. Results AK2 was expressed in the temporal lobes and hippocampuses of all epileptics, mainly in the cell bodies, and not in the brain tissues of the controls. Conclusion The overexpression of AK2 may be involved in the pathogenesis of drug-refractory epilepsy and become an important symbol thereof.