TNF—a和LSA浓度联合分析对肝癌诊断和鉴别诊断的临床价值

目的 :观察肿瘤坏死因子 -a( TNF-a)和脂质结合唾液酸 ( LSA)在慢性肝病中的变化及其临床价值。方法 :以酶联免疫吸附法 ( EL ISA)和化学抽提法 ,分别定量检测 16例慢性肝炎、16例肝硬化和 75例肝癌患者血清 TNF -a和 L SA浓度 ,并进行了比较分析。结果 :慢性肝病患者血清 TNF和 L SA浓度均增加 ,明显高于正常对照组 ( P<0 .0 1)。在慢性肝炎、肝硬化和肝癌患者中 ,TNF-a异常率分别为 3 1.3 %、43 .8%和 93 .3 % ;L SA异常率分别为 2 5 .0 %、6 .3 %和 73 .3 % ;在肝癌患者中两者的总阳性率达 97.3 %。结论 :慢性肝病时肝细胞合成的 TNF-a和 L SA增加 ,它们的联合检测有助于肝癌的诊断和鉴别诊断

CLINICAL VALUES OF COMBINED DETERMINATION OF TNF-a AND LSA IN DIAGNOSIS AND DIFFERENTIAL DIAGNOSIS OF HEPATOCELLULAR CARCINOMA

Objective :To explore the alteration and clinical values of tumor necrosis factor-a (TNF-a) and lipid associated sialic acid (LSA) in patients with chronic liver diseases. Methods: The levels of TNF-a and LSA in sera of patients with chronic hepatitis (CH), liver cirrhosis (LC) and hepatocellular carcinoma (HCC) were quantitated by enzyme-linked immunosorbent assay (ELISA) and chemical extraction assays, respectively. Results: The levels of TNF-a and LSA were significantly higher in patients with chronic liver diseases than those in normal controls (P<0.01). The positive rates of TNF-a and LSA abnormality were 31.3% and 25.0% in CH, 43.8% and 6.3% in LC, and 93.3% and 73.3% in HCC, respectively. The positive rate of combined determination of TNF-a and LSA was 97.3% in HCC. Conclusions: The present data suggest that the syntheses of TNF-a and LSA increase in hepatocytes of patients with chronic liver diseases, and the combined determination of TNF-a and LSA levels is helpful to diagnosis and differential diagnosis of HCC.