| 优化刺齿凤尾蕨抗癌二萜的超声提取工艺 |
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| 引用本文： | 范世锦,郑雅元,吕应年,苟占平. 优化刺齿凤尾蕨抗癌二萜的超声提取工艺[J]. 中国实验方剂学杂志, 2012, 18(11): 49-51 |
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| 作者姓名： | 范世锦 郑雅元 吕应年 苟占平 |
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| 作者单位： | 1. 广东医学院药学院,广东东莞523808;
2. 广东医学院药学院,广东东莞523808;广东医学院广东天然药物研究与开发重点实验室,广东湛江524023 |
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| 基金项目： | 广东省科技计划项目(20O9B03O8O1336);广东省东莞市高等院校科研机构科技计划项目(2008108101050) |
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| 摘 要： | 目的:优选刺齿凤尾蕨抗癌二萜的超声提取工艺.方法:采用L9(34)正交设计试验考察乙醇体积分数、提取时间、提取次数和料液比4个影响因素,以抗肿瘤活性二萜化合物Ent-11α-hydroxy- 15-oxo- kaur-16 -en-19 -oic -acid(5F)的含量为评价指标,采用HPLC进行含量测定.结果:超声提取最佳工艺为A2B2C3D3,即加12倍量75％乙醇超声提取3次,每次65 min.结论:优选得到的工艺稳定可行.
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| 关 键 词： | 刺齿凤尾蕨 抗癌 二萜 正交试验 超声波提取 |
| 收稿时间： | 2011-12-28 |
Optimization of Ultrasonic Extraction Process for Antitumor Diterpenoid from Pteris dispar |
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| FAN Shi-jin,ZHENG Ya-yuan,LV Ying-nian and GOU Zhan-ping. Optimization of Ultrasonic Extraction Process for Antitumor Diterpenoid from Pteris dispar[J]. China Journal of Experimental Traditional Medical Formulae, 2012, 18(11): 49-51 |
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| Authors: | FAN Shi-jin ZHENG Ya-yuan LV Ying-nian GOU Zhan-ping |
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| Affiliation: | College of Pharmacy, Guangdong Medical College, Dongguan 523808, China;College of Pharmacy, Guangdong Medical College, Dongguan 523808, China;College of Pharmacy, Guangdong Medical College, Dongguan 523808, China;Guangdong Key Lab for Research and Development of Natural Drugs, Guangdong Medical College, Zhanjiang 524023, China;College of Pharmacy, Guangdong Medical College, Dongguan 523808, China;Guangdong Key Lab for Research and Development of Natural Drugs, Guangdong Medical College, Zhanjiang 524023, China |
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| Abstract: | Objective:To optimize ultrasonic extraction technology of antitumor diterpenoid from Pteris dispar.Method:Four factors of the concentration of ethanol,extraction time,extraction times and solid-liquid ratio were investigated by L9(34) orthogonal design test,the content of ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid(5F) was defined as index,the content of 5F was determined by HPLC.Result:A2B2C3D3 was optimum ultrasonic extraction technology,which was ultrasonic extracted 3 times with 12 times the amount of 75% ethanol by 65 min each time.Conclusion:This optimized process was stable and available. |
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| Keywords: | Pteris dispar antitumor diterpenoid orthogonal test ultrasonic extraction |
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