| 痘苗病毒真核表达载体pMJ601的转化与鉴定 |
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| 引用本文： | 郜恒骏,朱红音,楼屹,顾伟齐,房静远,任卫平,萧树东. 痘苗病毒真核表达载体pMJ601的转化与鉴定[J]. 南京医科大学学报(自然科学版), 2000, 20(6): 481-483 |
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| 作者姓名： | 郜恒骏 朱红音 楼屹 顾伟齐 房静远 任卫平 萧树东 |
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| 作者单位： | [1]上海第二医科大学仁济医院消化科,上海市消化疾病研究所 [2]上海第二医科大学仁济医院消化科,上海市消化疾病研究 |
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| 基金项目： | 国家自然科学基金资助项目(39770345) |
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| 摘 要： | 目的 对痘苗病毒表达载体ｐＭＪ６０１进行转化与鉴定，为进一步实施痘苗病毒为载体的基因治疗作必要的准备。方法 利用感受态细胞的制备及转化、质粒抽提、琼脂糖凝胶电泳、限制性内切酶酶切等多种基因工程技术对质粒ｐＭＪ６０１进行转化及鉴定。结果 琼脂糖凝胶电泳清楚地显示了ｐＭＪ６０１质粒３条带和Ｂａｍ ＨＩ或Ｈｉｎｄ Ⅲ酶切后的线性条带。结论 痘苗病毒表达ｐＭＪ６０１的转化与鉴定，为痘苗病毒载体系统的基因治
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| 关 键 词： | 痘苗病毒 表达载体 pMJ601 基因工程 真核 |
| 修稿时间： | 0200-01-20 |
Transformation and Identification of Vaccinia Expression Vector pM J601 |
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| Gao Hengjun,Zhu Hongyin,Lou Yi,et al.. Transformation and Identification of Vaccinia Expression Vector pM J601[J]. Acta Universitatis Medicinalis Nanjing, 2000, 20(6): 481-483 |
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| Authors: | Gao Hengjun Zhu Hongyin Lou Yi et al. |
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| Affiliation: | Gao Hengjun,Zhu Hongyin,Lou Yi,et al. Department of Gastroenterology,Renji Hospital of Shanghai Second Medical University,Shanghai Institute of Digestive Disease,Shanghai 200001 |
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| Abstract: | Objective To transform and identify vaccinia expression vector, pMJ601, for the necessary equipment of gene therapy based on the vector of pox virus. Methods Genetic engineering techniques such as preparation and transformation of competent cell, plasmid extraction, agarose gel electrophoresis, restriction analysis, and some others were used to transform and identify pox virus expression vector pMJ601.Results There were three fragments in pMJ601, which were apparently shown by agarose gel electrophoresis.Conclusion It is one of the critical steps to further gene therapy based on pox virus. |
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| Keywords: | pox virus pMJ601 genetic engineering |
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