离体冻伤模型创面愈合过程中基质细胞衍生因子1促表皮干细胞迁移的研究

目的 了解离体皮肤冻伤模型创面愈合过程中,基质细胞衍生因子1(SDF-1)对创缘表皮干细胞(ESC)的运动趋化作用.方法 体外构建三维皮肤等价物全层冻伤模型(创面呈孔形),免疫组织化学染色观察冻伤后3、7 d创缘间质内SDF-1的表达情况.另将冻伤模型分为对照组(创面区添加PBS 50 μL/孔)、SDF-1组(创面区添加100 ng/mL SDF-1,50μL/孔)和AMD3100组创面区用100 ng/mL AMD3100(50μL/孔)处理30 min后,添加SDF-1 50μL/孔],观察各组创缘中ESC的分布变化.结果 免疫组织化学染色显示,冻伤后3、7 d创缘间质内SDF-1的表达逐渐增加.与对照组及AMD3100组相比,SDF-1组创缘基底层整合素β1阳性细胞数量增多,且部分阳性细胞向上层表皮迁移聚集,创缘ESC数量更多,表皮细胞层数增加.结论 SDF-1促进ESC向创缘迁移聚集参与创面修复,这可能是ESC参与创面修复过程的机制之一.

Promotion effect of stromal cell-derived factor 1 on the migration of epidermal stem cells in the healing process of frostbite-wound model ex vivo

Objective To study the promotion effect of stromal cell-derived factor 1 (SDF-1) on the migration of epidermal stem cells (ESC) in the healing process of frostbite-wound model ex vivo. Methods A three-dimensional model of full-thickness frostbite of skin was constructed (with slot-like wound) out of skin equivalent. The expression of SDF-1 in wound stroma was observed with immunohisto-chemistry staining on post injury days (PID) 3 and 7. The model frostbite wounds were divided into control group (treated with PBS 50 μL per wound) , SDF-1 group (treated with 100 ng/mL SDF-1, 50 μL per wound) , and AMD3100 group treated with 100 ng/mL AMD3100 (50 μL per wound) for 30 minutes, and then SDF-1 50 μL was added per wound]. The redistribution of ESC around wound was observed. Results The expression of SDF-1 in wound stroma increased gradually on PID 3 and 7. Compared with those in control and AMD3100 groups, there were more ESC and epithelial cell layers, and more integrin β1-positive cells appeared at the basal layer of wound in SDF-1 group, and some of the positive cells migrated upward to epidermis. Conclusions SDF-1 contributes to wound repair through promoting ESC to migrate toward and gather around wound edge. This may be one of the mechanisms of ESC participating in wound repair.