大鼠肝切除术后肝损伤程度与肝再生状态的动态对比研究

目的：探讨肝切除术后肝损伤程度与肝再生状态的关系。方法：Wistar大鼠随机分为三组：（1）假手术组；（2）正常大鼠肝切除组；（3）肝硬化大鼠肝切除组，建立大鼠肝切除模型，观察围手术期血清和肝组织匀浆液中丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）水平、肝重／体重、肝再生率，以及应用免疫组化方法（SABC法）检测肝组织中增殖细胞核抗原（PCNA）的表达。结果：与正常大鼠相比，肝切除后肝硬化大鼠血清ALT和AST水平升高显著，持续时间较长（P＜0．05）；PCNA在肝切除后肝组织的表达显著延迟（P＜0．05），血清ALT和AST水平与肝再生率呈显著负相关（P＜0．05）。结论：肝损伤程度和肝再生状态呈负相关，肝损伤的程度影响肝再生状态，肝切除后检测血清ALT、AST水平的变化可以间接的了解肝再生情况。

Dynamic comparison between degree of liver injury and state of liver regeneration after hepatectomy in rats

Objective To investigate the relationship between degree of liver injury and state of liver regeneration after hepatectomy. Methods Wistar rats were randomized into 3 groups: (1) sham operated group (group A);(2) normal rats receiving hepatectomy group (group B);(3) cirrhotic rats receiving hepatectomy group (group C). After operation in all the 3 groups, we determined the levels of aminotransferase in serum and liver tissue, ratio of liver weight to body weight, liver regeneration rate and expression of proliferating cell nuclear antigen (PCNA) in liver tissue. Results As compared with the normal rats, the level of aminotransferase in serum after hepatectomy was significantly higher and lasted longer in cirrhotic rats (P<0 05) and the expression of PCNA in liver tissue delayed and altered much slowlier (P<0 05). The levels of ALT and AST in the serum were negatively correlated to the liver regeneration (P<0 05). Conclusions The degree of liver injury is negatively correlated to and affects the state of liver regeneration. Detection of aminotransferase level after hepatectomy can indirectly reflect liver regeneration.