Ecdysteroids in adults of the nematode, Dirofilaria immitis |
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Authors: | A H Mendis M E Rose H H Rees T W Goodwin |
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Institution: | Department of Biochemistry, University of Liverpool, P.O. Box 147, Liverpool, L69 3BX England |
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Abstract: | Adult males and females of the dog heartworm, Dirofilaria immitis, were extracted separately and, following separation of the free and conjugated ecdysteroid fractions, the conjugates were hydrolysed enzymically. Both the ecdysteroids released by hydrolysis of the conjugates and the free hormones were further purified and analysed by a combination of radioimmunoassay, thin-layer chromatography and high-performance liquid chromatography monitoring fractions by radioimmunoassay, and by gas-liquid chromatography/mass spectrometry (selected ion monitoring). Both males and females contained free and conjugated ecdysteroids. Evidence was obtained for the presence of ecdysone, 20-hydroxyecdysone, 20,26-dihydroxyecdysone and possibly ponasterone A. The possible parallel between ecdysteroid endocrinology in nematodes and insects is discussed. |
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Keywords: | Moulting hormones Ecdysteroids Gas chromatography/mass spectrometry Selected ion monitoring GC/MS gas chromatography/mass spectrometry GLC gas-liquid chromatography HPLC high-performance liquid chromatography RIA radioimmunoassay SIM selected ion monitoring TLC thin-layer chromatography TMSI |
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