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FasL基因表达对缺氧状态下直肠癌细胞增殖凋亡的影响
引用本文:赵菲,李世拥,安萍,于波,蔡慧云. FasL基因表达对缺氧状态下直肠癌细胞增殖凋亡的影响[J]. 中华胃肠外科杂志, 2005, 12(1): 239-243. DOI: 10.3760/cma.j.issn.1671-0274.2009.03.010
作者姓名:赵菲  李世拥  安萍  于波  蔡慧云
作者单位:北京军区总医院全军普通外科中心,100700;
基金项目:国家自然科学基金全军科技攻关课题
摘    要:Objective To elucidate the effect of FasL gene expression on the proliferation and apoptosis of hypoxic rectal carcinoma cells. Methods The normoxic expression level of FasL in HR-8348 subtype cells (HR-8348B, HR-8348L, HR-8348F and HR-8348As) with different invasive power were verified by Western blot. Hypoxia models for HR-8348B, HR-8348L, HR-8348F and HR-8348As were constructed with chemical modeling, then the FasL levels in all groups at 12 h after hypoxia were quantitated by Western blot. Distribution of different cell life cycles was determined with flow cytometry. Cell reproductive activities were detected with MTT method, and cell apoptesis was assessed with TUNEL. Results FasL protein was pigmentized at the position of 40 000 by Western blot, and the expression level of FasL was significantly higher in HR-8348F cells than those in HR-8348B, HR-8348L and HR-8348As cells(F=361.149, P<0.01) in normoxia. At 12 h after hypoxia, the FasL level was also significantly higher in HR-8348F cells than those in other groups (F=278.766, P<0.01), but was not markedly different as compared to themselves in normoxia (t=1.762, P>0.05). The proliferation index was significantly higher in HR-8348F (60.43±3.72) than those in HR-8348B (40.01±3.30), HR-8348L (41.30±4.06) and HR-8348As cells (35.87±4.39), respectively (F=39.477, P<0.01). However, both inhibition rate of proliferation and apoptotic index were remarkably lower in HR-8348F (17.30±1.98 and 13.10±1.04) than those in HR-834B (33.70±4.33 and 21.60±1.31), HR-8348L (34.20±3.92 and 20.10±1.15), and HR-8348As (38.00±4.55 and 23.90±1.23), respectively (F=28.811 and 76.462, respectively, P<0.01). Conclusion The expression enhancement of intracellular FasL in rectal carcinoma in hypoxia can lead to accelerated proliferation and reduced apeptosis of cells, which will promote tumor cells to adapt microenvironmental hypoxia.

关 键 词:直肠肿瘤   基因   Fas配体   缺氧   细胞增殖   细胞凋亡   

Effect of FasL gene expression on proliferation and apoptosis of rectal carcinoma cells in hypoxia state
ZHAO Fei,LI Shi-yong,AN Ping,YU Bo,CAI Hui-yun. Effect of FasL gene expression on proliferation and apoptosis of rectal carcinoma cells in hypoxia state[J]. Chinese journal of gastrointestinal surgery, 2005, 12(1): 239-243. DOI: 10.3760/cma.j.issn.1671-0274.2009.03.010
Authors:ZHAO Fei  LI Shi-yong  AN Ping  YU Bo  CAI Hui-yun
Abstract:
Keywords:Rectal neoplasmsGene  Fas ligandAnoxiaCell proliferationApeptosis
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